在食品工业中使用生物工程酶和精密发酵
Fatma Boukid1, Seedhabadee Ganeshan2, Yingxin Wang2
1ClonBio Group Ltd., 6 Fitzwilliam Pl, D02 XE61 Dublin, Ireland.
International journal of molecular sciences
|June 28, 2023
概括
酶工程为食品加工创造定制的酶. 扩大这些设计酶的生产面临挑战,但精密发酵和固态发酵等方法提供了解决方案.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物技术是生物技术.
- 酵素工程是什么? 酶工程是什么
背景情况:
- 原生酶在食品加工中在活性,效率,基质范围和稳定性方面存在局限性.
- 酶工程通过创建具有增强性质的定制酶提供解决方案.
研究的目的:
- 审查酶工程策略,以开发改进的酶.
- 突出大规模酶生产面临的挑战和潜在解决方案.
主要方法:
- 讨论了酶工程方法:理性设计,定向进化,半理性设计.
- 探讨合成生物学,基因编辑,人工智能和精密发酵的计算分析方面的进展.
- 考虑了扩大规模的挑战,转基因微生物的安全性,无细胞系统和固态发酵 (SSF).
主要成果:
- 在现代生物技术工具的帮助下,酶工程使得能够创建具有卓越功能的设计酶.
- 精密发酵为这些工程酶提供了高效的生产途径.
- 扩大规模仍然是一个瓶,安全性和成本效益是关键考虑因素.
结论:
- 尽管酶工程和精密发酵技术取得了进展,但扩大生产规模存在重大障碍.
- 无细胞系统和固态发酵 (SSF) 是有利于成本效益和更安全的酶生产的有希望的替代方案.
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