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在MCF-7L细胞中,IGF-1刺激了糖溶性ATP的产生

Bhumika Rajoria^1,2, Xihong Zhang², Douglas Yee^1,2,3

¹Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA.

International journal of molecular sciences

|June 28, 2023

概括

胰岛素样生长因子1 (IGF-1) 受体,而不是胰岛素受体,调节乳腺癌细胞中的ATP产生. 这一发现澄清了IGF系统在癌症代谢中的作用.

科学领域:

生物化学生物化学
分子生物学分子生物学
癌症研究癌症研究

背景情况:

胰岛素类生长因子 (IGF) 系统与乳腺癌的进展有关.
复杂性和受体同质性 (胰岛素受体[IR]和1型IGF受体[IGF-1R]) 阻碍了治疗向.
IGF系统影响细胞增殖和新陈代谢,使其成为研究的关键途径.

研究的目的:

通过量化实时ATP生产来研究乳腺癌细胞的代谢表型.
确定IGF-1R和IR在细胞代谢调节中的特定作用.

主要方法:

使用的MCF-7L和抗他莫西芬的MCF-7L (MCF-7L TamR) 乳腺癌细胞系.
在用胰岛素类生长因子1 (IGF-1) 和胰岛素进行急性刺激后,实时测量ATP生产率.
在具有不同IGF-1R和IR表达水平的细胞中比较ATP生产.

主要成果:

刺激IGF-1 (5nM) 在MCF-7L细胞中显著增加了糖溶性ATP的产生.
胰岛素 (10nM) 没有改变MCF-7L细胞的新陈代谢.
无论IGF-1还是胰岛素都没有影响MCF-7L TamR细胞中的ATP产生,这些细胞表现出下调的IGF-1R.

结论:

在这些乳腺癌模型中,IGF-1R,而不是IR,是ATP产生的主要调节者.
这项研究强调了IGF轴,代谢功能障碍和乳腺癌之间的直接联系.
研究结果表明,针对IGF-1R的特定向可能是调节癌症代谢的可行策略.

关键词:

乳腺癌乳腺癌乳腺癌在这种情况下,胰岛素胰岛素.胰岛素受体的胰岛素受体代谢失调是代谢失调的原因之一.1型胰岛素样生长因子 1型胰岛素样生长因子受体

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