使用基于皮肤的先进NGS技术的整体方法,评估山羊的气候适应力的新见解
Silpa Mullakkalparambil Velayudhan1, Veerasamy Sejian2,3, Chinnasamy Devaraj3
1Institute of Animal Breeding and Genetics, Justus Liebig University Giessen, Ludwigstr. 21b, 35390 Giessen, Germany.
International journal of molecular sciences
|June 28, 2023
概括
热应激显著影响山羊毛和皮肤特征,改变基因表达和微生物群. 科迪阿杜山羊表现出比卡尼阿杜山羊更大的热弹性,这是由于基因组和分子变化较少.
科学领域:
- 动物科学动物科学
- 基因组学就是基因组学.
- 环境生理学环境生理学
背景情况:
- 热应激对畜牧业生产构成重大挑战,影响动物健康和生产力.
- 土著山羊品种具有独特的适应能力,但它们对热应激的反应需要详细的研究.
- 了解特定品种的热弹性对于在气候变化中可持续的畜牧管理至关重要.
研究的目的:
- 阐明热应激对两种土著山羊品种头发和皮肤特征的多方面的影响.
- 在表型和基因组层面上对Kanni Aadu和Kodi Aadu山羊的热弹性进行比较评估.
- 确定与山羊热应激反应和适应相关的关键分子和生理标记.
主要方法:
- 使用气候室对Kanni Aadu和Kodi Aadu山羊进行模拟热应激研究.
- 对表型特征的全面分析:头发特征,头发皮质醇,出汗率和皮肤组织测量.
- 基因组和分子分析:毛囊qPCR,皮肤元基因组学 (16S rRNA),转录组学和二硫酸盐测序.
主要成果:
- 热应激显著改变了头发纤维的长度,并诱导了热冲击蛋白的表达 (HSP70,HSP90,HSP110).
- 热应激的山羊表现出增加的出汗率,激活的汗腺和皮肤结构的改变.
- 观察到皮肤微生物群,基因表达 (DEGs) 和DNA甲基化 (DMRs) 的显著变化,Kanni Aadu显示出比Kodi Aadu更大的改变.
结论:
- 与Kanni Aadu山羊相比,科迪阿杜山羊表现出优越的热弹性,其体现出对热应激的基因组和分子反应不那么明显.
- 热应激会在多个层面影响山羊皮肤组织,从物理特征到复杂的分子通路.
- 这项研究提供了关于特定品种适应热应激的宝贵见解,为将来的山羊育种和管理策略提供了信息.
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