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全基因组DNA甲基化和转录基因组集成关联DNA甲基化变化与由葡萄球菌染色体引起的牛子临床乳腺炎
Mengqi Wang1,2, Nathalie Bissonnette1, Mario Laterrière3
1Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC J1M 0C8, Canada.
International journal of molecular sciences
|June 28, 2023
概括
DNA甲基化变化与乳牛中由葡萄球菌染色体 (SCM) 引起的亚临床乳腺炎有关. 这些表观遗传变化可能调节宿主免疫反应,并作为乳腺健康的潜在生物标志物.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 兽医医学 兽医医学 兽医医学
- 微生物学 微生物学
背景情况:
- * * Staphylococcus chromogenes * (SC) 是乳制品农场新出现的乳腺炎病原体.
- *亚临床乳腺炎 (SCM) 影响乳制品生产和动物福利.
- *DNA甲基化在SCM中的作用在很大程度上仍未被探索.
研究的目的:
- * 研究SCM中全基因组DNA甲基化模式.
- *为了将甲基化变化与牛奶体细胞中的基因表达相关联.
- * 为了确定SCM的潜在DNA甲基化生物标志物.
主要方法:
- * 全基因组双硫酸盐测序用于甲基组分析.
- *RNA测序用于转录组概况.
- *生物信息学整合甲基组和转录组数据.
主要成果:
- * 在SCM中发现了显著的DNA甲基化变化 (DMCs,DMRs,dMHBs).
- *调节区域中的DNA甲基化与基因表达有负相关.
- *1486个基因表现出与免疫功能相关的甲基化和表达变化.
- *16个dMHB被确定为SCM的潜在歧视性签名.
结论:
- *大量的DNA甲基化变化与SCM有关.
- *表观遗传修饰可能在调节宿主对SC的免疫反应中发挥作用.
- *已识别的dMHBs显示为SCM和乳腺健康的诊断生物标志物的潜力.
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