综合转录基因组和代谢基因组分析揭示了"夏季黑色"葡萄中初级芽的致病因子
Shaogang Fan1, Yanshuai Xu1, Miao Bai1
1College of Horticulture, Hunan Agricultural University, Changsha 410128, China.
International journal of molecular sciences
|June 28, 2023
概括
葡萄树中初级芽亡是由反应性氧物种 (ROS) 引起的细胞损伤引起的. 这项研究表明,ROS的积累会破坏蛋白质质量,导致死亡和产量减少.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 初级芽亡是一种影响葡萄产量的显著生理障碍,特别是在亚热带双重作物系统中.
- 目前尚不清楚主要的致病机制和初级芽亡的有效解决方案.
- 本研究侧重于"夏季黑"葡萄品种,以阐明这种疾病的进展和分子基础.
研究的目的:
- 为了研究葡萄芽中初级芽亡的进展和不可逆转性.
- 为了识别与初级芽缩相关的分子和代谢变化.
- 探索潜在的调节因素和涉及初级芽亡的途径.
主要方法:
- 使用染色和传输电子显微镜观察初级芽亡的进展.
- 在死发育过程中对冬季芽的综合转录组和代谢组分析.
- 分析反应性氧物种 (ROS) 积累及其下游影响.
主要成果:
- 主要芽亡的启动包括等离子体溶解,线粒体胀和器官损伤.
- ROS的积累破坏了细胞蛋白质质量控制,导致线粒体和内分泌网膜的压力.
- 代谢从黄酸盐转变为酸盐发生,乙烯增加和奥素信号发生变化,可能导致亡.
结论:
- 活性氧物种诱导的细胞损伤,包括线粒体功能障碍和蛋白质聚合,是初级芽亡的主要原因.
- 代谢变化和荷尔蒙失衡 (乙烯,奥素) 在葡萄死亡的发展中起着至关重要的作用.
- 了解这些机制为管理初级葡萄芽亡和提高葡萄产量提供了潜在的目标.
关键词:
在VvP231中使用.葡萄藤是一种葡萄.初级芽缩的主要原因蛋白质的错误折叠 蛋白质的错误折叠有活性氧物种的反应性氧物种.组织棕色化 组织棕色化转录组 (transcriptome) 是一个转录组.更多相关视频
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