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使用数字全息显微镜进行定量阶段成像,以评估性结肠炎患者的肠炎症程度

Arne Bokemeyer¹, Joost Buskermolen², Steffi Ketelhut³

¹Department of Gastroenterology, Hepatology and Transplant Medicine, University Hospital of Essen, University Duisburg-Essen, 45147 Essen, Germany.

Journal of clinical medicine

|June 28, 2023

概括

数字全息显微镜 (DHM) 为评估性结肠炎 (UC) 炎症提供了一个客观的方法. 这项研究发现,基于DHM的折射率可靠量化UC组织学活性,与已建立的评分系统相关.

科学领域:

胃肠病学胃肠病学
医疗成像医学成像
病理学病理学病理学

背景情况:

性结肠炎 (UC) 涉及慢性结肠直肠炎症,使组织学缓解成为关键的治疗目标.
由于众多的评分系统和需要专门的专业知识,评估UC组织病理学具有挑战性.
定量相成像 (QPI) 和数字全息显微镜 (DHM) 提供了在组织中客观的,无污点的炎症量化.

研究的目的:

评估DHM用于定量评估UC患者体质病理性炎症.
为了将DHM衍生的亚皮质折射指数 (RI) 与已确定的组织学,内镜和UC中的临床发现相关联.

主要方法:

从21名UC患者的结肠和直肠粘膜活检使用基于DHM的QPI进行了分析.
从DHM图像中提取了皮下折射指数 (RI).
RI数据与南希指数 (NI),梅奥内镜分值 (MES) 和临床结果相关联.

主要成果:

在基于DHM的RI和南希指数之间发现了显著的相关性 (R2 = 0.251,p < 0.001).
RI值也与梅奥内镜子评分相关 (R2 = 0.176,p < 0.001).
一个RI切线1.3488显示了84%的灵敏度和72%的特异性来识别活跃的UC.

结论:

在性结肠炎中,DHM为定量评估粘膜炎症提供了可靠的工具.
来自DHM的亚皮质折射率是区分活跃UC和非活跃疾病的强有力的参数.

关键词:

数字全息显微镜的数字全息显微镜.组织病理学组织病理学这是一种炎症炎症炎症炎症.炎症性肠病是一种炎症性肠病.定量阶段成像成像技术折射率指数的折射率指数是什么性结肠炎是一种

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