从Lactobacillus johnsonii MG释放的GAPDH通过调节与紧密结合相关的基因来增强屏障功能
Mengying Lyu1, Yuying Bai1, Kanami Orihara1
1School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Microorganisms
|June 28, 2023
概括
来自Lactobacillus johnsonii MG的细胞外甘-3-酸脱酶 (GAPDH) 通过与结合粘附分子-2 (JAM-2) 结合来修复受损的肠紧结. 特定的GAPDH序列被确定为JAM-2相互作用和细菌细胞结合.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 生物化学 生化学
背景情况:
- 来自Lactobacillus johnsonii MG的细胞外甘-3-酸盐脱酶 (GAPDH) 与肠道上皮成分相互作用,包括结合粘附分子-2 (JAM-2).
- 在此之前,GAPDH在增强Caco-2细胞紧密结的确切作用和特异性尚不清楚.
研究的目的:
- 为了研究MG衍生的Lactobacillus johnsoniiGAPDH对Caco-2细胞紧接处再生的影响.
- 确定负责JAM-2相互作用和细菌细胞表面结合的特定GAPDH片段.
主要方法:
- 评估GAPDH对Caco-2细胞中过氧化诱导的紧接点损伤的影响.
- 使用高性能液态染色学 (HPLC) 和飞行时间质谱学 (TOF-MS) 净化和预测相互作用的GAPDH序列.
- 分子对接模拟以分析-JAM-2相互作用.
主要成果:
- GAPDH特异地与JAM-2结合,并在Caco-2细胞中恢复了H2O2受损的紧接点,从而导致基因上调.
- 两个特定的GAPDH,11GRIGRLAF18 (N端) 和323SFTCQMVRTLLKFATL338 (C端),与JAM-2显著相互作用和对接.
- 预计一个单独的 (52DSTHGTFNHEVSATDDSIVVDGKKYRVYAEPQAQNIPW89) 将结合到细菌细胞表面.
结论:
- 乳杆菌 johnsonii MG GAPDH在促进损坏的肠道紧接处的再生方面发挥着新的作用.
- GAPDH的特定N端和C端序列对于JAM-2结合至关重要,而其他序列则调解细菌细胞相互作用.
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