免疫学和微生物组分析的试点横截面研究揭示了吸烟者,电子烟使用者和从未吸烟者的明显炎症概况
Peter G Shields1,2, Kevin L Ying1,3, Theodore M Brasky1,2
1Comprehensive Cancer Center, The Ohio State University and James Cancer Hospital, Columbus, OH 43210, USA.
Microorganisms
|June 28, 2023
概括
吸烟和电子烟会增加肺部不分化的M0巨细胞. 虽然两者都影响炎症基因表达,但吸烟者与电子烟使用者和从未吸烟者相比,表现出明显的差异.
科学领域:
- 肺免疫学 肺免疫学
- 微生物组研究 微生物组研究
- 毒理学 毒理学 毒理学
背景情况:
- 吸烟者表现出肺部免疫细胞数量增加和炎症基因表达.
- 电子烟使用也会影响肺部免疫反应.
- 了解这些影响对于公共卫生至关重要.
研究的目的:
- 为了调查吸烟,电子烟使用和肺部微生物组组成之间的联系.
- 分析与吸烟状况相关的免疫细胞亚型和炎症基因表达.
- 探索肺部微生物群,免疫细胞和炎症之间的相关性.
主要方法:
- 在28名参与者身上进行了支气管镜检查和支气管膜洗.
- 用RNA测序和CIBERSORT算法来分析免疫细胞和基因表达.
- 转录组学分析了肺部微生物组的组成.
主要成果:
- 与从未吸烟者相比,吸烟者和EC用户的M0巨细胞增加了两倍,M2巨细胞减少.
- 在吸烟者和从未吸烟者 (68个基因),吸烟者和EC使用者 (19个基因) 之间观察到炎症基因表达的显著差异.
- 对于每个参与者群体,都确定了不同的肺免疫和炎症概况,与微生物组变化有有限的相关性.
结论:
- 吸烟和EC使用与M0巨细胞的增加和肺部炎症基因表达的改变有关.
- 与EC使用者和从不吸烟者相比,吸烟者表现出独特的炎症基因表达特征.
- 该研究表明,吸烟和EC使用对炎症的毒性肺部影响,可能独立于微生物组变化.
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