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通过新细菌菌株完成迪克洛芬雅克的生物降解:假设的途径和降解酶

Mahmoud S M Mohamed¹, Ayan A Asair¹, Nashwa A H Fetyan²

¹Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt.

|June 28, 2023

概括

研究人员确定了四种细菌菌株,能够降解持久性药物迪克洛菲纳克. 阿克罗巴克特西班牙菌S11实现了97.79%的降解,为废水处理提供了一个有前途的生物催化剂.

科学领域:

环境微生物学环境微生物学
生物修复是一种生物修复.
制药污染制药污染制药污染

背景情况:

像迪克洛芬雅克这样的异生菌化合物在生态系统中积累,造成环境风险和毒性.
狄克洛芬雅克的持久性是由于其缓慢的自然降解和高毒性,需要有效的去除策略.

研究的目的:

为了分离能够降解二二的细菌.
在狄克洛芬雅克生物降解过程中识别中间代谢物.
为了确定参与狄克洛芬雅克降解途径的酶.

主要方法:

隔离和选择使用狄克洛芬雅克作为唯一碳来源的细菌菌株 (高达40毫克/升).
优化生长条件,以降解狄克洛芬雅克.
使用标准微生物技术识别细菌分离物.
通过高性能液体染色学 (HPLC) 来量化二二烯酸的降解.
使用气体染色学质谱法 (GC-MS) 分析生物降解代谢物.
对关键分离物的酶活性测定 (乳酶,过氧酶,二氧化酶).

主要成果:

选择了四种细菌分离物,这些细菌分离物被确定为Pseudomonas aeruginosa (S1),Alcaligenes aquatilis (S2),Achromobacter spanius (S11) 和Achromobacter piechaudii (S18),这些细菌分离物被发现.
阿克罗巴克特西班牙菌S11在6天后表现出最高的降解效率 (97.79±0.84%).
在所有测试的分离物中观察到二甲的初始氧化,环裂被认为是通过P. aeruginosa S1和A. piechaudii S18进行完全生物降解的关键步骤.
酶试验表明,乳糖酶,过氧酶和二氧化原酶在某些菌株的降解过程中参与.

结论:

这项研究成功地分离并鉴定出强大的二甲降解细菌,突出了Achromobacter spanius S11作为一种高效的菌株.
了解代谢途径和酶机制为开发生物修复策略提供了基础.
这些发现支持开发细菌生物催化剂,以有效地从污染水中去除药物,促进水的再利用.

关键词:

这种细菌是Achromobacter.伪omonas 是一个类型的.生物降解生物降解狄克洛芬雅克 (Diclofenac) 是一种可怕的药物.代谢物的代谢物.

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