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在不同压力条件下由突变菌株的细菌生物膜形成

Margarita Sharipova¹, Natalia Rudakova¹, Ayslu Mardanova¹

¹Institute of Fundamental Medicine, Kazan Federal University, Kremlevskaya St. 18, 420008 Kazan, Russia.

|June 28, 2023

概括

细菌细菌生物膜的形成受环境因素和遗传调节的影响. 缺少蛋白酶的菌株和abrB等特定突变物增强生物膜发育,而 degU等其他菌株则减少生物膜发育.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生化学

背景情况:

细菌细菌是一种促进植物生长的草原细菌 (PGPR),以其在植物根上形成生物膜的能力而闻名.
了解影响生物膜形成的因素对于优化其在农业和工业中的有益应用至关重要.

研究的目的:

调查环境条件 (温度,pH值,盐,氧化应激,金属离子) 和基因突变对Bacillus subtilis生物膜形成的影响.
分析细胞外蛋白酶和特定调节基因 (degU,abrB,spo0A) 在生物膜发育和矩阵组成中的作用.

主要方法:

在不同的环境条件下培养Bacillus subtilis WT 168,调节性突变和蛋白酶缺乏菌株.
量化生物膜形成水平.
使用共聚焦显微镜检查生物膜矩阵结构和粉样蛋白质含量.

主要成果:

细菌素168在22°C-45°C的温度范围内形成强大的生物膜,pH值为6-8.5,对盐和氧化应激有耐受性.
双价离子Ca2+,Mn2+和Mg2+促进生物膜的形成,而Zn2+则抑制它.
缺少蛋白酶的菌株和abrB突变体表现出增强的生物膜形成,deGU突变体表现出减少的生物膜形成,而spo0A突变体表现出生物膜水平的动态变化.
突变和缺少蛋白质酶的菌株表现出改变的生物膜矩阵结构,在 degU 突变和缺少蛋白质酶的菌株中含有更高的粉样蛋白质含量.

结论:

环境因素和特定的遗传突变显著调节细菌细菌生物膜的形成和矩阵特性.
细胞外蛋白酶和调节基因,如deGU,abrB和spo0A,在控制生物膜发育中起着至关重要的作用.
这些发现提供了关于细菌细菌生物膜复杂调节的见解,这对利用其PGPR能力具有重要意义.

关键词:

B. subtilis 的意思,就是说.同焦点显微镜的共焦显微镜.金属离子金属离子缺乏蛋白酶的菌株.监管突变变异的人盐的压力是盐的压力.

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