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鉴定Bacillus pumilus菌株与针对性基因编辑用于抗菌和分泌因子的特征

Iuliia V Danilova¹, Iuliia A Vasileva¹, Ajgul I Gilmutdinova¹

¹Research Laboratory "Agrobioengineering", Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia.

|June 28, 2023

概括

菌菌菌株 3-19 株

科学领域:

微生物学微生物学
植物病理学植物病理学
遗传学遗传学是一个

背景情况:

细菌物种产生对植物有益的抗微生物.
了解Bacillus pumilus 3-19的对抗机制对于农业应用至关重要.

研究的目的:

调查特定基因在Bacillus pumilus 3-19的对抗活性中的作用.
为了表征B. pumilus 3-19衍生物与非活化的和分泌基因.

主要方法:

使用CRISPR-Cas9基因组编辑来禁用B. pumilus 3-19中的bacilysin (bac),bacteriocin (bact) 和sigF基因.
评估了对B. cereus和Pantoea brenneri的对抗活性.
分析了突变菌株的生长动态和蛋白质分解活性.

主要成果:

对bac,bact和sigF基因的无活化减少了抗菌活性,特别是对抗bacilysin.
突变菌株表现出改变的生长动态和减少的蛋白质分解活性.
由于sigF的非激活,导致了异原性B. pumilus 3-19突变.

结论:

巴西利辛在B. pumilus 3-19对土壤微生物的对抗作用中发挥着独特的作用.
针对性基因组编辑为微生物对抗的遗传基础提供了洞察力.
B. pumilus 3-19的衍生品显示了植物保护相关的修饰性质.

关键词:

菌菌 (Bacillus pumilus) 是一种菌.这就是CRISPR-CAS9的作用.具有对抗性的活动.抗微生物类抗微生物.球胞形成因子是分泌因子.

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