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在Rhodococcus sp. 中编码长链基氧酶的新基因的功能分析. 菌株CH91 菌株CH91 菌株
Wei Xiang1, Shan Hong1, Yanfen Xue1
1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Microorganisms
|June 28, 2023
概括
两个基因,alkB1和alkB2,来自Rhodococcus sp. CH91菌株对于降解长链n-基 (C16-C36) 是至关重要的. 在这种n-alkane生物降解过程中,alkB2比alkB1发挥更重要的作用.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 罗多科克 (Rhodococcus sp.) 是一种植物. 菌株CH91使用长链n-基作为唯一的碳来源.
- 全基因组分析预测了两个AlkB型基化酶基因:alkB1和alkB2.
研究的目的:
- 为了确定alkB1和alkB2在n-alkane降解中的功能性作用,使用CH91.1.
- 调查alkB1和alkB2对碳化合物代谢的差异性贡献.
主要方法:
- 使用RT-qPCR进行基因表达分析.
- 基因淘汰实验以评估功能影响.
- 在Pseudomonas fluorescens KOB2Δ1.1.中异性基因表达.
主要成果:
- 无论是alkB1还是alkB2基因都是由n-基因 (C16-C36) 诱导的,alkB2显示出更高的上调.
- 淘汰突变体在n-基上表现出较低的生长和降解率,而alkB2突变体表现出更明显的效果.
- 在P. fluorescens中,alkB1或alkB2的异质表达恢复了烯降解活性.
结论:
- 基B1和基B2都对Rhodococcus sp.中C16-C36 n-基的降解至关重要. CH91. 一个人的心.
- 在n-代谢中,AlkB2的作用比AlkB1.1的作用更为重要.
- 这些基因是工程细菌生物修复石油碳化合物污染的有希望的候选人.
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