代谢工程,以有效地生产Z,Z-Farnesol在E. 大肠杆菌
Mengyang Lei1,2, Zetian Qiu1,2, Leilei Guan1,2
1Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, China.
Microorganisms
|June 28, 2023
概括
研究人员利用大肠杆菌产生Z,Z-farnesol (Z,Z-FOH),这是一种用于化品和制药的有价值化合物. 这种代谢工程努力实现了迄今为止最高的微生物Z,Z-FOH标位,使生物合成成为可能.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 生物技术是生物技术.
背景情况:
- Z,Z-farnesol (Z,Z-FOH), farnesol 的全化异构体,在化品,日常化学品和药品方面具有显著的潜力.
- 目前的生产方法可能是有限的,需要替代的可持续生物合成途径.
研究的目的:
- 通过代谢工程*Escherichia coli*进行*de novo*生产Z,Z-farnesol.
- 识别和优化参与Z,Z-farnesol生物合成途径的关键酶.
主要方法:
- 选五种Z,Z-farnesyl二酸盐 (Z,Z-FPP) 合成酶,以催化尼里二酸盐到Z,Z-FPP.
- 对Z,Z-FPP去化到Z,Z-FOH的十三种酸酶的评估.
- *cis*-prenyltransferase的局部定向突变发生,以增强Z,Z-FOH的产生.
主要成果:
- 改造的大肠杆菌成功地产生了Z,Z-FOH.
- 一种最佳的突变菌株通过批量发酵实现了572.13 mg/L的Z,Z-FOH标位.
- 这代表了Z,Z-FOH的最高报告的微生物标位.
结论:
- 这项研究报告了首次在大肠杆菌中*de novo*生物合成Z,Z-farnesol.
- 开发的代谢工程策略为生产Z,Z-FOH和其他cis*-terpenoids提供了基础.
- 工程 *大肠杆菌 *显示承诺作为一个细胞工厂,可持续的类生产.
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