通过转基因 Pseudomonas sp. 生产多基酸盐 phDV1:对利用葡萄酒行业废物作为碳源进行比较研究
Athina Drakonaki1, Eirini Mathioudaki1, Ermis Dionysios Geladas1
1Department of Chemistry, University of Crete, GR-70013 Voutes, Greece.
Microorganisms
|June 28, 2023
概括
这项研究设计了Pseudomonas sp. phDV1通过敲除PHA脱聚合酶 (phaZ) 和调节蛋白 (phaR) 来增强多基酸盐 (PHA) 的产生. 发酵酶突变体显示出增加的聚-3-基酸盐 (PHB) 产量,特别是与,这表明具有成本效益的工业应用.
科学领域:
- 微生物生物技术 微生物生物技术
- 生物聚合物生产 生物聚合物生产
- 合成生物学 合成生物学
背景情况:
- 伪omonas sp. 这种病毒是假的. 在phDV1中,自然会产生多基基酸 (PHAs).
- 内源PHA脱聚合酶 (phaZ) 和调节蛋白 (phaR) 限制了细菌PHA的积累.
- 对PHA生产途径的基因改造对于工业应用至关重要.
研究的目的:
- 为了构建Pseudomonas sp.的phaZ和phaR淘汰突变体. phDV1.1. phDV1.1. phDV1.1. 这是一个很好的方法. phDV1.1.
- 为了研究和量化这些突变体中的PHA生产,使用和葡萄残渣作为基质.
- 评估具有成本效益的工业PHA生产潜力.
主要方法:
- 使用基因工程技术构建phaZ和phaR淘汰突变体.
- 野生类型和突变菌株的培养,使用和葡萄末.
- 通过光显微镜选PHA生产.
- 使用高性能液体染色学 (HPLC) 量化PHA (聚-3-基酸盐 - PHB).
- 使用1H核磁共振 (NMR) 分析确认PHB的结构.
主要成果:
- 成功构建了phaZ和phaR淘汰突变体.
- 野生类型在48小时内使用葡萄末生产了~280μgPHB/g细胞.
- 发酵酶突变在72小时内产生了约310μg的PHB/g细胞,特别是在的存在下.
- 证实PHA是聚-3-基酸盐 (PHB).
结论:
- 消除phaZ显著增强了Pseudomonas sp.中的PHB积累. phDV1.1. phDV1.1. phDV1.1. 这是一个很好的方法. phDV1.1.
- Z突变体使用醇合成高水平PHB的能力表明了具有成本效益的生物聚合物生产的潜力.
- 这项研究为优化微生物PHB制造工艺铺平了道路.
关键词:
PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB伪omonas sp. 这种病毒是假的. 这种菌株是phDV1在 phaR 敲击中.在 phaZ 淘汰赛中.聚基基酸盐的多基基酸盐更多相关视频
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