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一种自动化固体相提取-UPLC-MS/MS方法,用于同时确定环境水中的硫胺抗菌剂

Mengyu Qi^1,2, Pengfei He², Hongmei Hu^2,3

¹Institute of Marine and Fisheries, Zhejiang Ocean University, Zhoushan 316021, China.

Molecules (Basel, Switzerland)

|June 28, 2023

概括

一种新方法可以准确地检测水中的17种硫胺 (抗生素). 这种先进的技术,使用液体染色学和质谱学,对于监测这些广泛使用的药物所带来的环境和健康风险至关重要.

科学领域:

环境化学环境化学
分析化学分析化学
药理学药理学是指药理学的学科.

背景情况:

在人类和兽医中广泛使用硫胺抗微生物药物对环境和健康构成重大风险.
水源被硫胺污染需要敏感且可靠的检测方法.

研究的目的:

开发和验证一种可靠的方法,用于在各种水矩阵中同时确定17种硫胺.
建立一种灵敏,高效的分析技术,用于对硫胺残留物的环境监测.

主要方法:

使用超高性能液体染色学-并联质谱法 (UHPLC-MS/MS) 同时确定17种硫胺.
完全自动化的固体相提取 (SPE) 用于样品制备和丰富.
使用了17个同位素标记的内部标准来减轻矩阵效应并确保准确性.

主要成果:

优化方法实现了高丰富系数 (982-1033),运行时间为6个样本,运行时间约为60分钟.
证明了出色的线性 (0.05-100μg/L),高灵敏度 (检测极限为0.01-0.05 ng/L),以及令人满意的恢复率 (79-118%).
在河水和海水样本中分别成功检测出六和七种硫胺,其中硫甲醇是最常见的.

结论:

使用自动化SPE开发的UHPLC-MS/MS方法是一种简单,强大和敏感的工具,用于分析各种水类中的硫胺.
该方法为评估水生环境中的硫胺污染提供了关键数据,并为公共卫生战略提供了信息.
这些发现凸显了天然水体中硫胺残留物的存在,强调了持续环境监测的必要性.

关键词:

自动固体相提取自动化固体相提取环境环境水环境水同位素稀释的同位素稀释硫胺抗菌剂硫胺抗菌剂超高性能液体染色学并发质谱学

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