普罗西奥可纳对Scenedesmus obliquus的酶选择性毒性作用
Qingqing Xiang1, Ying Zhou1,2, Chengxia Tan1
1College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
Molecules (Basel, Switzerland)
|June 28, 2023
概括
杀菌剂prothioconazole (PTC) 对Scendesmus obliquus.藻类表现出对抗选择性毒性. 该S-(+) -PTC反体比R-(-) -PTC更有毒,影响藻类生长和细胞完整性.
科学领域:
- 环境毒理学环境毒理学
- 生态毒理学 生态毒理学
- 水生物质毒理学研究
背景情况:
- 蛋白甲醇 (PTC) 是一种广泛的三醇杀菌剂.
- PTC存在两个反体:R-(-) -PTC和S-(+) -PTC.
- 了解对抗选择性毒性对于环境风险评估至关重要.
研究的目的:
- 为了研究prothioconazole对淡水藻类Scendesmus obliquus的对抗选择性毒性作用.
- 为了比较PTC赛马体 (Rac-PTC) 和其单个反体的毒性.
主要方法:
- 对S. obliquus进行了急性毒性测试,这些S. obliquus暴露在不同度的Rac-PTC,R-(-) -PTC和S-(+) -PTC中.
- 确定了EC50的值.
- 测量包括生长比率,光合作用色素含量,酶 (CAT) 和酶活动,甲酸 (MDA) 水平和细胞形态分析.
主要成果:
- PTC反体和赛马体表现出剂量依赖的毒性.
- 72小时的EC50值为Rac-PTC的8.15 mg·L−1,R-(-) -PTC的16.53 mg·L−1和S-(+) -PTC的7.85 mg·L−1.
- 与R-(-) -PTC相比,S-(+) -PTC诱导了CAT和酶活动的更大的抑制和更高的MDA水平.
- 细胞形态破坏和膜损伤在S-(+) -PTC和Rac-PTC中比R-(-) -PTC更为明显.
结论:
- 在Scendesmus obliquus中观察到大量的prothioconazole对抗选择性毒性.
- 该S-(+) -PTC反体表现出更高的毒性,导致对藻类生长,生理学和细胞完整性的更严重影响.
- 这些发现凸显了在对PTC等性农药的生态风险评估中考虑enantioselectivity的重要性.
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