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评估的抗氧化能力的比较方法:试图解释差异的尝试
Vanessa B Paula1,2, Letícia M Estevinho2,3, Susana M Cardoso4
1Doctoral School, University of León (ULE), Campus de Vegazana, 24007 León, Spain.
Molecules (Basel, Switzerland)
|June 28, 2023
概括
这项研究强调了不同的方法如何影响的抗氧化剂和含量分析. 方法的选择和特定的化合物,如基酸显著影响结果.
科学领域:
- 分析化学 分析化学
- 自然产品 化学 化学
背景情况:
- 蜂是一种蜜蜂产品,其生物活性归因于其复杂的和黄成分.
- 了解的成分对于其在健康和工业中的应用至关重要.
研究的目的:
- 分析葡萄牙的花粉状况,总含量 (TPC),抗氧化特性和化合物.
- 为了比较TPC和抗氧化能力的不同分析方法的有效性.
- 调查化合物与测量活动之间的相关性.
主要方法:
- 使用六种方法量化TPC:folin-Ciocalteu (F-C),光谱光度计 (SPECT) 和电压计 (SWV).
- 通过DPPH,FRAP和铁酸盐测定 (OFec,MFec) 评估抗氧化能力.
- 使用UHPLC-DAD-ESI-MS.识别和定量化合物.
主要成果:
- 谱光测量 (SPECT) 给出了最高的TPC值 (422 ± 98毫克GAE/g),而SWV给出了最低的 (47 ± 11毫克GAE/g).
- 改性铁化物 (MFec) 试验显示了最高的抗氧化能力,其次是DPPH.
- 主要识别的化合物包括素,咖啡酸异烯酸,平诺布林,加兰,平诺素-3-O-乙酸和咖啡酸烯酸.
结论:
- 分析方法的选择显著影响TPC和中的抗氧化剂活性量化.
- 基酸 (HBA) 和基酸 (HCA) 含量是的TPC和抗氧化能力的关键贡献者.
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