在流通的鸟类外周血液单核细胞中WNV的细胞内多样性揭示了宿主依赖的多重感染模式
Dalit Talmi-Frank1, Alex D Byas1, Reyes Murrieta1
1Center for Vector-Borne Infectious Diseases, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Pathogens (Basel, Switzerland)
|June 28, 2023
概括
乌中高西尼罗病毒 (WNV) 病毒病允许细胞内比红更大的病毒遗传多样性. 乌的多样性增加可能解释了WNV群体动态和健康状况的宿主特异性差异.
科学领域:
- 病毒学 病毒学
- 进化生物学 进化生物学
- 免疫学 免疫学 免疫学
背景情况:
- 关节动物传播的病毒 (arboviruses),如西尼罗河病毒 (WNV),表现出宿主依赖的种群动态.
- 美国乌表现出高WNV遗传多样性和弱净化选择,与美国红不同,病毒性较低.
- 红中WNV的传递增强了健康状况,而乌中的传递则没有.
研究的目的:
- 为了测试乌的高病毒素是否支持单个鸟类外周血液单核细胞 (PBMC) 内的更大的遗传多样性.
- 研究 WNV 遗传多样性和适应性中宿主特异性差异背后的机制.
主要方法:
- 鸟类细胞和鸟类感染分子条形码的WNV.
- 从单个细胞中测序病毒RNA以量化WNV条形码丰富度.
- 在乌和红之间比较WNV种群多样性和变异维护.
主要成果:
- 乌中的WNV种群丰富度明显超过了红中观察到的WNV种群丰富度.
- 乌比红更频繁地维持罕见的WNV变种.
- 乌的病毒血量较高与维护有缺陷的基因组和不太普遍的变异相关,可能是通过补充.
结论:
- 乌的病毒病增加导致PBMC中的WNV遗传多样性较高,与红相比.
- 宿主易感性,以病毒性水平为特征,影响WNV群体的多样性和演变.
- 乌中较弱的净化选择可能是由于较高的病毒血症,多种感染和补充,影响了WNV的演变.
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