米子微生物减轻慢性不可预测的轻度压力诱导的肌肉缩
Sosorburam Batsukh1,2, Seyeon Oh2, Kyoungmin Rheu3
1Department of Anatomy & Cell Biology, College of Medicine, Gachon University, Incheon 21936, Republic of Korea.
Nutrients
|June 28, 2023
概括
用g-aminobutyric acid (RG) 补充的米芽有效地对抗慢性压力诱导的肌肉缩. 通过调节关键信号通路,RG使皮质醇水平正常化,并保护肌肉质量和功能.
科学领域:
- 内分泌学 在内分泌学.
- 肌肉生理学 肌肉生理学
- 营养科学 营养科学
背景情况:
- 慢性压力导致下丘脑-垂体-上腺 (HPA) 轴的失调,从而提高皮质醇水平.
- 较高的葡萄糖皮质醇 (GCs) 诱导肌肉降解并抑制合成,导致肌肉缩.
- 确定应激诱导的肌肉缩的有效干预措施对于保持健康至关重要.
研究的目的:
- 为了研究用30%的γ-aminobutyric acid (RG) 补充米芽的有效性,以减轻肌肉缩.
- 评估RG对HPA轴荷尔蒙和GC信号传递在慢性不可预测轻度应激 (CUMS) 模型中的影响.
- 阐明RG对肌肉的保护作用背后的分子机制.
主要方法:
- 建立一个CUMS动物模型来诱导慢性压力.
- 用RG来评估其对上腺体体重,ACTH和皮质醇水平的影响.
- 分析GC受体 (GR) 表达和结合,肌肉降解/合成途径标记,氧化应激标记,以及肌肉特异性参数 (体重,纤维面积,握力).
主要成果:
- CUMS显著增加了上腺体重,ACTH和皮质醇水平,这些都被RG扭转了.
- RG减弱了CUMS诱导的GR表达和结合,抑制了肌肉降解途径 (Klf15,Redd-1,FoxO3a,Atrogin-1,MuRF1) 和增强的肌肉合成途径 (IGF-1/AKT/mTOR/s6k/4E-BP1).
- RG减少了CUMS诱导的氧化应激 (iNOS,乙化p53),改善了细胞增殖,并恢复了肌肉重量,纤维面积和握力.
结论:
- 补充RG有效地减弱了HPA轴激活和由CUMS引起的皮质醇相关肌肉缩.
- RG通过调节GC信号,抑制降解途径,促进合成途径和减少氧化应激来保护骨肌肉.
- RG显示出作为一种治疗药物的潜力,用于管理压力诱导的肌肉损耗.
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