胆通过miR-129-5p调节SOX4,并在发育皮层中修改H3K27me3
Evan M Paules1, Jorge A Silva-Gomez1, Walter B Friday1
1Department of Nutrition, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
Nutrients
|June 28, 2023
概括
胆的可用性通过调节神经前代细胞影响大脑发育. 低胆摄入量通过miR-129-5p减少SOX4蛋白,影响细胞增殖和分化.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 胆对于神经前体细胞 (NPC) 在发育中的大脑皮层的增殖和分化至关重要.
- 胆可用性影响这些过程的精确分子机制仍然不完全理解.
研究的目的:
- 阐明通过胆可用性调节发育大脑中的NPCs的分子途径.
- 确定关键的分子参与者,包括转录因子和microRNAs,参与胆依赖的神经发生.
主要方法:
- 研究了胆可用性对NPC中SOX4蛋白水平的影响.
- 分析了微RNA-129-5p (miR-129-5p) 在调节SOX4表达中的作用.
- 对miR-129-5p.p.进行了功能增益和功能丧失测定.
- 在发育中的皮质中评估了全球H3K27me3水平.
主要成果:
- 在神经发生过程中低胆摄入量会降低NPC中的SOX4蛋白水平.
- 这种减少是由miR-129-5p的异常表达介导的,而不是降解的增加.
- 降低SOX4和EZH2 (下游目标) 导致H3K27me3减少,影响NPC的扩散和分化.
结论:
- 胆的可用性通过miR-129-5p调节NPC中的转录因子SOX4.
- 这种营养介导的途径影响表观遗传修饰 (H3K27me3) 和关键的发育过程,如细胞增殖和分化.
- 介绍了在大脑发育过程中对基因表达的营养调节的新机制.
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