来自纳米颗粒的过渡性涂层实现了宽频和高抗菌性能
Rachel Zaia1, Giovanna M Quinto1, Livia C S Camargo1
1Biocolloids Laboratory, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Avenida Professor Lineu Prestes, 748, Butantan, São Paulo 05508-000, Brazil.
Pharmaceuticals (Basel, Switzerland)
|June 28, 2023
概括
使用纳米颗粒 (NP) 的新型阴离子涂层显示出对细菌和真菌的广泛抗微生物活性. 这些短暂的涂层为生物医学材料提供了潜力.
科学领域:
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
- 微生物学 微生物学
背景情况:
- 开发有效的抗微生物涂层对于预防生物医学应用中的感染至关重要.
- 基于纳米粒子的涂层为抗微生物表面修饰提供了新的策略.
- 对于某些生物医学材料来说,暂时的抗微生物活性是可取的,以防止长期殖民.
研究的目的:
- 描述和评估玻璃上的阴离子和性纳米粒子涂层的抗菌活性.
- 研究聚二甲 (PDDA) 和格拉米西丁D (Gr) 纳米颗粒对常见病原体的疗效.
- 了解开发的涂料的作用机制和剂量反应关系.
主要方法:
- 用PDDA和Gr纳米颗粒的二层化碎片 (BF) 被成并干燥在玻璃盖片上.
- 使用殖民地形成单位 (CFU) 计数,对Pseudomonas aeruginosa,Staphylococcus aureus和Candida albicans进行了定量评估.
- 研究了涉及静电附着和细胞膜破坏的相互作用机制.
主要成果:
- 含有PDDA和Gr纳米颗粒的涂层显示出显著的广谱抗菌活性,将细菌和真菌活力降至零.
- 在低剂量PDDA (5μg) 和Gr (0.94μg) 或更高剂量 (25μg PDDA,4.6μg Gr) 的情况下,可以达到最佳活性.
- 抗微生物作用是暂时的,因为涂层在干燥后被洗掉,使表面变得非抗微生物.
结论:
- PDDA和Gr纳米颗粒的组合可以创建有效的,广泛的抗菌涂层.
- PDDA和Gr的协同作用通过破坏微生物细胞壁和膜来增强抗菌效果.
- 这些短暂的抗微生物涂层对生物医学材料的应用具有前景,在这些材料中需要临时的抗微生物特性.
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