墨西哥原生黑豆富含安东的提取物调节与炎症相关的生物标记物
Jonhatan Contreras1, Montserrat Alcázar-Valle1, Eugenia Lugo-Cervantes1
1Food Technology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Unidad Zapopan, Camino Arenero 1227, El Bajío del Arenal, Zapopan 45019, Jalisco, Mexico.
Pharmaceuticals (Basel, Switzerland)
|June 28, 2023
概括
本地黑豆产生强大的抗氧化剂和抗炎性质的富含安托素的提取物 (ARE). 这些提取物,特别是超临界流体提取物 (RE) 和一个分量 (REF2),对营养药和功能性食品应用有前途.
科学领域:
- 食品科学与技术 食品科学与技术
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 安西是植物中发现的有力生物活性化合物.
- 本地黑豆是一种丰富的安东素来源.
- 了解它们的抗氧化和抗炎潜力对于食品和健康应用至关重要.
研究的目的:
- 从原生黑豆中获取和描述富含安东素的提取物 (ARE).
- 评估这些提取物及其分片的抗氧化和抗炎活性.
- 探索本土黑豆作为营养药和功能性食品来源的潜力.
主要方法:
- 使用Amberlite® XAD-7树脂进行超临界流体提取 (RE),然后进行净化 (PE).
- 使用逆流色谱分离提取物,得到四个分数 (REF1,REF2,PEF1,PEF2).
- 提取物和分量的表征,以及抗氧化剂 (ABTS,DPPH) 和抗炎症 (NO,COX-1,COX-2,iNOS) 潜力的评估,包括理论结合能量分析.
主要成果:
- 观察到显著的抗氧化活性,ABTS IC50值在7.9至139.2毫克C3GE/L之间,DPPH IC50值在9.2至117.2毫克C3GE/L之间.
- 证明了强大的抗炎作用,NO IC50值从0.6到143.8毫克C3GE/L,COX-1 IC50从0.1到0.9毫克C3GE/L,COX-2 IC50从0.01到0.7毫克C3GE/L,和iNOS IC50从0.9到5.6毫克C3GE/L.
- 超临界流体提取物 (RE) 和其分量REF2表现出最高的抗氧化和抗炎潜力,理论结合能量表明与炎症标的良好相互作用.
结论:
- 反流色谱在隔离和净化黑豆提取物中的生物活性化合物方面是有效的,同时保留了它们的生物活性.
- 原生黑豆具有有吸引力的植物化学特征,并具有显著的抗氧化和抗炎性质.
- 这些发现支持使用本地黑豆作为开发营养药和功能性食品的有价值成分.
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