Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Herpes01:28

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
Genital Herpes01:23

Genital Herpes

Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Emulsion-based synthesis of polycaprolactone/bioactive glass 45S5 microparticles for bone regeneration applications.

Biomedical materials (Bristol, England)·2026
Same author

Retraction notice to"Nitric oxide sensing by chlorophyll a" [Anal. Chim. Acta 985 (2017) 101-113].

Analytica chimica acta·2026
Same author

Reproducible detection of antigen-specific T cells and Tregs via standardized and automated activation-induced marker assay workflows.

Cell reports methods·2026
Same author

Revolutionizing vision using corneal 3D printing.

Trends in biotechnology·2026
Same author

Insights into the regulation and signaling landscape of cardiotrophin-like cytokine factor 1 (CLCF1).

Biochimica et biophysica acta. Molecular cell research·2026
Same author

MPN-FIT: a randomized controlled pilot trial of supervised exercise in myeloproliferative neoplasms.

Blood advances·2026

相关实验视频

Updated: Jun 24, 2026

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection
07:55

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

Published on: November 3, 2012

12.9K

在HSV-1感染的子角膜中含有酸胆和KR12的角膜植入物

Kamal Malhotra1,2, Oleksiy Buznyk3,4, Mohammad Mirazul Islam3

  • 1Department of Ophthalmology, Université de Montréal, Montreal, QC H3C 3J7, Canada.

Pharmaceutics
|June 28, 2023
PubMed
概括

有KR12的生物合成植入物显示出治疗HSV-1眼部感染的潜力. 这些植入物促进角膜愈合和神经再生,为受影响个体的视力恢复提供希望.

关键词:
这是一种HSV-1感染.KR12 KR12 KR12 KR12 KR12 KR12 KR12 KR12 KR12 KR12 KR12 KR12 KR12 KR12 KR12在RHCIII-MPC中.角膜植入物的角膜植入物纳米颗粒是一种纳米粒子.子 子 子 子复兴再生是一种再生方式.

更多相关视频

Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK
07:27

Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK

Published on: December 18, 2012

12.0K
Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals
08:31

Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals

Published on: September 20, 2021

3.3K

相关实验视频

Last Updated: Jun 24, 2026

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection
07:55

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

Published on: November 3, 2012

12.9K
Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK
07:27

Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK

Published on: December 18, 2012

12.0K
Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals
08:31

Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals

Published on: September 20, 2021

3.3K

科学领域:

  • 眼科医生 眼科 眼科
  • 生物材料科学 生物材料科学
  • 病毒学 病毒学

背景情况:

  • 疹简单病毒1型 (HSV-1) 感染可能导致严重的角膜损伤和失明.
  • 在感染HSV-1的个体中,由于移植失败率高,角膜移植通常是禁忌的.
  • 需要新的生物材料来抑制炎症并促进角膜再生.

研究的目的:

  • 评估无细胞生物合成植入物 (RHCIII-MPC) 结合KR12释放纳米颗粒,用于治疗感染HSV-1的角膜.
  • 评估复合植入物的抗炎和再生能力,在体外和体内.
  • 确定KR12在阻断病毒活性和促进伤口愈合方面的有效性.

主要方法:

  • 开发了RHCIII-MPC植入物,其中含有装载KR12.2的二氧化纳米粒子.
  • 在实验室中评估了KR12对HSV-1的细胞毒性和抗病毒活性.
  • 在HSV-1感染的子角膜中通过前层叶片角质整形评估植入物的性能.
  • 在6个月内监测病毒载量,炎症,新血管化和组织再生.

主要成果:

  • KR12在体外显示出细胞友好性,并阻断HSV-1活动,促进上皮细胞伤口关闭.
  • 复合植入物在体外释放KR12长达3周.
  • 在体内,植入物没有减少病毒载荷或炎症,但确实减少了病毒传播.
  • 在6个月的时间里,观察到角膜上皮,侧膜和神经的稳定再生.

结论:

  • 结合KR12的生物合成植入物显示出对治疗HSV-1角膜感染的前途.
  • 植入物促进角膜组织再生和神经修复,尽管持续的炎症.
  • 进一步的研究可能会优化这些植入物,以改善抗炎作用和视力恢复.