复合材料基于聚乙烯 (ε-caprolactone) 和用Oligo/Poly (Glutamic Acid) 修改的石墨烯氧化物作为具有骨导性质的生物材料
Olga Solomakha1, Mariia Stepanova1, Iosif Gofman1
1Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg 199004, Russia.
Polymers
|June 28, 2023
概括
这项研究开发了用聚胺酸修饰的石墨烯氧化物 (GO) 用于增强可生物降解的骨再生生物材料. 新的复合材料改善了机械性能,刺激了细胞生长,显示了骨组织工程的前景.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 纳米技术纳米技术
背景情况:
- 骨组织再生需要具有骨传导性质的先进生物降解生物材料.
- 石墨烯氧化物 (GO) 是一个有前途的材料,但其修改是增强功能的关键.
- 聚氨基酸 (Glu) 提供生物相容性和潜在的骨质导电性.
研究的目的:
- 开发一种新的途径,用藻/多聚胺酸 (oligo/poly ((Glu)) 来修改氧化石墨烯 (GO)).
- 制造和鉴定PCL基复合材料的特征,其中包括改性GO.
- 评估用于骨再生的开发生物材料的机械性能,细胞毒性和骨质生成潜力.
主要方法:
- 石墨烯氧化物 (GO) 已被修改为oligo/poly(glutamic 酸 (oligo/poly(Glu)).
- 使用里埃变换红外光谱,HPLC,热重力测量分析,SEM和光散射技术证实了修改.
- 用修改的GO制造的聚乙烯 (PCL) 复合膜被制造并测试了机械性能,细胞毒性 (MG-63细胞) 和细胞增殖 (hMSCs). 骨质导电性通过性酸酶试验和染色 (素,阿利沙林红色S) 进行评估.
主要成果:
- 通过多种分析方法证实了GO与oligo/poly (Glu) 的成功修改.
- 与PCL/GO复合材料相比,PCL复合材料与改性GO复合材料的弹性模量增加了18-27%
- 在人类骨髓瘤细胞 (MG-63) 中没有观察到显著的细胞毒性.
- 复合材料刺激了人类介质干细胞 (hMSC) 的增殖,并在试验室中证明了骨传导性.
结论:
- 由oligo/poly (Glu) 修改的开发的GO是创造增强生物降解的骨再生生物材料的可行组件.
- 基于PCL的复合材料表现出更好的机械强度和生物相容性.
- 这些新型生物材料显示出通过增强骨质生成促进骨组织再生的巨大潜力.
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