在Bacillus subtilis的子上显示的TonB依赖受体蛋白刺激了对Acinetobacter baumannii的保护性免疫反应
Nor-Aziyah MatRahim1,2,3, Kathryn Marie Jones2, Brian P Keegan2
1Tropical Infectious Diseases Research and Education Center (TIDREC), Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Vaccines
|June 28, 2023
概括
研究人员使用Bacillus subtilis子开发了一种新的口服疫苗,以显示Acinetobacter baumannii TonB依赖的受体. 这种方法成功地产生了对抗性细菌的免疫反应和杀菌活性.
科学领域:
- 微生物学 微生物学
- 疫苗学 疫苗学 疫苗学
- 免疫学 免疫学 免疫学
背景情况:
- 抗生素耐药的 *Acinetobacter baumannii* 构成了全球严重的健康威胁.
- 有限的治疗选择需要新的疫苗策略.
- B依赖受体 (TBDR) 是针对*A. baumannii*的疫苗的潜在目标.
研究的目的:
- 研究 *Bacillus subtilis* 子表现TBDRs 作为口服疫苗候选人对抗*A. baumannii* 的潜力.
- 在小鼠模型中评估复合子的免疫性和疗效.
主要方法:
- 工程 * Bacillus subtilis * 子以显示 * Acinetobacter baumannii * TBDRs 在它们的表面.
- 将重组子口服给小鼠进行疫苗接种.
- 评估了幽默和粘膜抗体的反应.
- 评估了血清对 *A. baumannii* 的临床分离物的杀菌活性.
主要成果:
- 用复合子口服接种疫苗在小鼠中没有引起疾病.
- 观察到对疫苗抗原有显著的粘膜和幽默抗体反应.
- 接种疫苗的小鼠血清对 *A. baumannii* 临床分离物具有杀菌活性.
结论:
- 在 *B. subtilis* 子上显示 TBDRs 是开发口服疫苗的一个有希望的策略.
- 子显示的TBDR引起了对*A. baumannii*的保护性免疫反应.
- 对这个平台的进一步探索是有必要的,因为对*A. baumannii*非常需要的疫苗.
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