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相关概念视频

Viral Mutations00:36

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A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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相关实验视频

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Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
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病毒群-通过蛋白质序列模拟扩大病毒数据库.

Julia Kende1, Massimiliano Bonomi2, Sarah Temmam3

  • 1Bioinformatics and Biostatistics Hub, Institut Pasteur, Université Paris Cité, F-75015 Paris, France.

Viruses
|June 28, 2023
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概括
此摘要是机器生成的。

病毒Pop模拟真实的病毒蛋白序列,以改进元基因组工具. 这个管道通过挑战分类学赋值和扩大用于检测新型病毒的数据库来增强病毒发现.

关键词:
氨基酸替代率是指氨基酸的替代率.我们的数据库数据库数据库数据库.人类基因组学学科.序列进化演变的过程序列模拟模拟的时间序列.

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科学领域:

  • 病毒学 病毒学
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 超基因组工具对于识别未知的病毒至关重要,但通常使用已知的序列进行评估.
  • 目前的评估方法限制了对工具检测新型或与之远距离相关的病毒的能力的评估.
  • 扩大公共数据库以现实的模拟序列可以改善对具有有限表征的病毒的检测.

研究的目的:

  • 介绍Virus Pop,一种用于模拟现实的蛋白质序列和家族遗传树的新型管道.
  • 加强对病毒发现的元基因组工具的基准测试.
  • 通过扩大序列数据库来改善新型和与之远距离相关的病毒的检测.

主要方法:

  • 病毒Pop模拟蛋白质序列与特定域的替换率变化.
  • 该管道推断出祖先序列,并将新的模拟序列插入到家族遗传树中.
  • 该工具使用sarbecoviruses的尖端蛋白和检测新型人类循环病毒进行了验证.

主要成果:

  • 病毒Pop生成模拟序列,准确地反映真实病毒蛋白的结构和功能特性.
  • 模拟序列与真实蛋白质序列非常接近,包括那些不在公共数据库中存在的蛋白质序列.
  • 该管道成功帮助识别了一种新型致病性人类循环病毒.

结论:

  • 病毒流行为挑战和改进元基因组学中的分类学赋值工具提供了一种有价值的方法.
  • 该管道可以增强病毒数据库,从而更好地检测远程和以前未知的病毒.
  • 病毒流行有助于描述元基因组数据的"暗物质".