鸟类传染性支气管炎病毒 (IBV) GI-23在巴西的演变和流行传播
Nilo Ikuta1, Diéssy Kipper1, Dayana Soriano Spencer de Freitas2
1Simbios Biotecnologia, Cachoeirinha 94940-030, RS, Brazil.
Viruses
|June 28, 2023
概括
传染性支气管炎病毒 (IBV) GI-23血统迅速蔓延到巴西,2018年左右有两次独立引入. 这项研究追踪了巴西肉养殖场的流行传播和遗传演变.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 禽类的健康 禽类的健康
背景情况:
- 传染性支气管炎病毒 (IBV) 是一个重要的全球家禽病原体.
- GI-23血统已经显示出快速的国际传播.
- 最近,IBV GI-23在巴西肉养殖场被发现,需要进行流行病学调查.
研究的目的:
- 调查IBV GI-23在巴西的引入和流行传播情况.
- 分析巴西IBV GI-23菌株的遗传多样性和进化动态.
- 为了识别与巴西IBV GI-23亚类相关的特定遗传标记.
主要方法:
- 在2021年10月至2023年1月期间,采集了94只感染IBV GI-23的肉群的样本.
- 使用实时RT-qPCR检测IBV GI-23的检测.
- 测序S1基因高变区1和2 (HVR1/2) 和完整的S1基因.
- 核酸序列数据的植物遗传学和植物动力学分析.
主要成果:
- 巴西IBV GI-23菌株形成了两个不同的亚分类 (SA.1和SA.2).
- 遗传学分析表明,IBV GI-23在2018年左右两次独立地引入巴西,可能来自东欧.
- 病毒种群动态显示,从2020-2021年增加,随后稳定和下降.
- 在HVR1/2中,特定的氨基酸替代体标志着巴西的SA.1和SA.2亚类.
结论:
- 最近,IBV GI-23被引入巴西,正在流行性地蔓延.
- 遗传数据支持这个血统的多个独立引入.
- 巴西IBV GI-23菌株的独特遗传概况为其正在进行的演变和传播提供了洞察力.
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