在中国中部分离的新型PRV变种的基因组表征和gE/gI删除菌株构造
Jianle Ren1, Shanshan Tan1, Xinxin Chen2
1College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Viruses
|June 28, 2023
概括
中国的新型伪狂热病毒 (PRV) 变种显示出显著的遗传变异. 一种新的突变疫苗在小鼠中表现出与Bartha-K61相似的保护,这表明了改善PRV控制策略的潜力.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 控制和控制猪病 控制和控制猪病
背景情况:
- 自2011年以来,伪狂犬病病毒 (PRV) 变种在中国的猪业造成了重大经济损失.
- 监测PRV野外菌株的遗传变异对于有效的疾病管理至关重要.
研究的目的:
- 从中国山西省分离的两种新型PRV变种菌株 (SX1910和SX1911) 的特征.
- 分析基因变异,包括关键蛋白和糖蛋白的突变.
- 在小鼠模型中评估新型PRV候选疫苗的疗效.
主要方法:
- 在PRV分离物SX1910和SX1911的全基因组测序.
- 遗传学分析和序列对齐以确定遗传变异.
- 蛋白质结构建模用于分析gB和gD糖蛋白中的氨基酸突变.
- 使用CRISPR/Cas9.9构建一个缺乏gE和gI基因的PRV SX1911突变体.
- 用突变病毒和Bartha-K61接种小鼠疫苗,然后用SX1911.11挑战.
主要成果:
- PRV变种表现出广泛的遗传变异,特别是在蛋白质编码序列UL5,UL36,US1和IE180.0中.
- 在糖蛋白gB和gD中发现了新的氨基酸突变,其中许多位于蛋白质表面.
- 接种SX1911-ΔgE/gI疫苗的小鼠表现出与Bartha-K61疫苗接种相似的保护.
- 与突变疫苗相比,Bartha-K61接种导致了较低的中和标位,更高的病毒载荷和更严重的病变,与SX1911挑战相比.
结论:
- 持续监测PRV遗传变异对于中国的猪健康至关重要.
- 新型PRV突变疫苗显示出希望,强调需要更新疫苗开发和疫苗接种策略.
- 了解PRV演变对于有效的控制计划和减轻猪业经济损失至关重要.
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