从高病毒载荷临床样本中直接对人类细胞巨乳病毒基因组进行纳米孔测序
Kathy K Li1,2, Betty Lau1, Nicolás M Suárez1
1Medical Research Council, University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK.
Viruses
|June 28, 2023
概括
纳米孔测序可以直接从临床样本准确地确定人类细胞巨乳病毒 (HCMV) 基因组. 这种方法绕过了丰富和放大,为HCMV提供了一个快速而精确的诊断工具.
科学领域:
- 基因组学就是基因组学.
- 病毒学 病毒学
- 生物信息学是一种生物信息学.
背景情况:
- 纳米孔测序提供了可移植性和实时分析,使其适合临床诊断.
- 最初的高错误率已经被硬件和软件的进步所缓解.
- 人类细胞巨乳病毒 (HCMV) 基因组测序对于诊断和疫情调查至关重要.
研究的目的:
- 评估纳米孔测序,以完成HCMV基因组的确定.
- 评估高病毒负载临床样本的可行性,而无需进行丰富或放大.
- 与已建立的测序方法相比,以确定精度.
主要方法:
- 混合生物信息学方法: de novo组装,读取对齐和序列抛光.
- 临床样本 (尿液和肺) 的直接测序,具有不同的HCMV负载.
- 通过Illumina测序生成的基因组与基准基因组进行比较.
主要成果:
- 实现了基因组基因组的高标识:99.97% (尿液) 和99.93% (肺部).
- 在没有病毒DNA丰富或PCR的情况下证明了成功的HCMV基因组确定.
- 在不同的病毒载量和样本类型中验证了准确性.
结论:
- 纳米孔测序是一种可行的方法,可以直接从临床样本中准确地确定HCMV基因组.
- 这项技术对快速,经济高效的诊断和疫情监测充满希望.
- 纳米孔测序的进一步进展增强了其在病毒学中的临床实用性.
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