温度对Cryphonectria低病毒的影响 1 它的真菌宿主,栗子虫病原体 Cryphonectria parasitica 中的积累和恢复
Pedro Romon-Ochoa1, Olivia Smith1, Alex Lewis2
1Forest Research, Plant Pathology Department, Alice Holt Research Station, Surrey GU104LH, UK.
Viruses
|June 28, 2023
概括
使用低毒性,Cryphonectria parasitica的病毒感染,对栗子病的生物控制是有效的. 在20-25°C之间,Cryphonectria hypovirus 1 (CHV1) 的存在显著降低了真菌生长,在15-25°C时病毒的最佳积累.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 病毒学 病毒学
背景情况:
- 栗子病是由寄生菌Cryphonectria寄生菌引起的,是栗子树的重大威胁.
- 使用低毒性进行生物控制,这种现象由诸如Cryphonectria hypovirus 1 (CHV1) 等真菌病毒介导,提供了一个有前途的管理策略.
- CHV1是Hypoviridae家族的类型物种,因其控制C. parasitica的作用而得到了广泛的研究.
研究的目的:
- 为了研究温度对英国C. parasitica. 隔离物中CHV1感染的影响.
- 量化CHV1积累及其对不同温度下真菌生长率的影响.
- 为了确定CHV1活动和恢复的最佳温度范围.
主要方法:
- 六个温度 (5-30°C) 被测试在六个受感染的C.寄生虫分离物 (三个具有CHV1菌株E-5,三个具有L-18) 和他们的非感染的对照.
- 实验是在马乳糖 (PDA) 和纤维素上进行的,每隔离物和温度使用三个复制品.
- 使用定量逆转录PCR (RT-qPCR) 来测量病毒度 (每微升纳米或复制数).
主要成果:
- 菌类生长速度受到温度的显著影响,显示出正相关性.
- 在20°C和25°C之间,CHV1的存在对C. parasitica生长有显著的负面影响.
- 温度严重影响CHV1积累和从寒冷或热应激中恢复,估计最佳范围为15-25°C.
结论:
- 温度在调节CHV1积累及其对C. parasitica的低病毒效应方面发挥着至关重要的作用.
- 对于CHV1活性及其对栗病的生物控制潜力的最佳温度范围在15°C至25°C之间.
- 了解这些温度动态对于有效部署基于CHV1的生物控制策略至关重要.
关键词:
这种病毒是Cryphonectria hypovirus 1病毒.这就是积累积累的过程.菌生长 菌生长 菌生长这是一种低毒性 (hypovirulence).恢复恢复恢复恢复的时间温度的温度的温度的温度的温度.更多相关视频
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