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通过C1q和C4b结合蛋白对SARS-CoV-2感染的补充激活-独立衰减

Nazar Beirag¹, Praveen M Varghese^1,2, Martin Mayora Neto³

¹Biosciences, College of Health, Medicine and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.

|June 28, 2023

概括

补充蛋白C1q和C4b结合蛋白 (C4BP) 通过抑制病毒进入和减少炎症,独立于补充激活,提供对SARS-CoV-2的保护. 这些蛋白质与尖蛋白结合,限制了肺部的感染和炎症反应.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
分子生物学分子生物学

背景情况:

补体系统对于对病毒的天生的免疫力至关重要.
严重的SARS-CoV-2感染包括过度的补体激活和细胞因子风暴.
补充蛋白C1q和C4b结合蛋白 (C4BP) 可能对病毒感染有保护作用.

研究的目的:

调查C1q和C4BP对抗SARS-CoV-2感染的补充激活独立作用.
检查C1q和C4BP与SARS-CoV-2尖端蛋白及其受体结合域 (RBD) 的直接相互作用.
评估C1q和C4BP对SARS-CoV-2细胞进入和相关免疫反应的影响.

主要方法:

指导ELISA检查蛋白相互作用.
RT-qPCR用于评估免疫反应调节.
使用伪型颗粒进行细胞结合和病毒进入试验.

主要成果:

C1q和C4BP通过尖端蛋白的RBD直接与SARS-CoV-2伪型粒子结合.
C1q和C4BP可以减少病毒与宿主细胞的结合和转导.
用C1q和C4BP治疗降低了包括IL-1β,IL-8,IL-6,TNF-α,IFN-α和RANTES在内的促炎性细胞因子和化学因子的mRNA水平.
C1q和C4BP治疗减少了SARS-CoV-2伪型感染介导的NF-κB激活.

结论:

当地产生的C1q和C4BP可独立于补体激活,对SARS-CoV-2感染提供保护.
这些蛋白质抑制病毒与宿主细胞的结合,并减弱炎症反应.
C1q和C4BP代表了管理SARS-CoV-2感染的潜在治疗点.

关键词:

在C1qq中,C1q为C1q.在C4BP中使用.在 COVID-19 疫情中,这就是SARS-CoV-2病毒.经典的路径是古典的路径.补充补充补充补充补充补充补充.这是先天免疫力.

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