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在SARS-CoV-2的替代病毒中和化试验中减少了Omicron B.1.1.529和人类ACE2受体之间的结合

Tove Hoffman¹, Linda Kolstad¹, Dario Akaberi¹

¹Department of Medical Biochemistry and Microbiology, Zoonosis Science Center (ZSC), Uppsala University, 752 37 Uppsala, Sweden.

|June 28, 2023

概括

一种新的SARS-CoV-2代理病毒中和测试 (sVNT) 检测到没有活病毒的中和抗体 (NAbs). 这种基于Luminex的测试为研究和公共卫生提供了传统病毒中和测试的安全和有效替代方案.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
诊断试验开发诊断试验开发

背景情况:

传统的病毒中和试验 (cVNT) 是检测SARS-CoV-2中和抗体 (NAbs) 的标准,但需要BSL-3实验室和传染性病毒.
这为广泛的诊断使用带来了重大的后勤和安全挑战.

研究的目的:

开发和验证使用Luminex技术的SARS-CoV-2代理病毒中和试验 (sVNT).
为NAb检测提供一个更安全,更容易获得的cVNT替代方案.

主要方法:

该sVNT的设计是为了模仿人类血管酶转化酶2 (hACE2) 受体与SARS-CoV-2尖峰 (S) 蛋白之间的相互作用.
该测试检测到武汉,三角洲和Omicron变种的这种相互作用的抗体阻塞.
对于高通量检测,采用了Luminex技术.

主要成果:

sVNT与cVNT显示出100%的定性相关性.
该试验显示了 hACE2与Omicron (B.1.1.529) 变体的S1域,S1+S2三元体和受体结合域 (RBD) 之间的结合减少.
这表明Omicron变种的受体结合效率可能较低.

结论:

开发的SARS-CoV-2sVNT是一种可靠和高效的诊断工具.
它在研究和公共卫生应用中作为cVNT的有价值替代品.
该试验的结果还提供了对Omicron变体的结合性特征的见解.

关键词:

在ACE2中,ACE2是ACE2.这就是Omicron.这就是SARS-CoV-2病毒.通过抗体介导的阻塞.受体结合领域是受体结合领域.斯派克斯派克斯派克斯就是一个尖刺.

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