相关实验视频

Updated: Jul 25, 2025

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

条件细胞重编程和空气-液体接口建模瘤病毒 (HPV和EBV) 在上皮细胞和病毒相关的人类癌症中的生命周期

Abdul Qawee Rani¹, Dilber Nurmemet¹, Joseph Liffick¹

¹Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA.

|June 28, 2023

概括

条件细胞重编程 (CCR) 和空气-液体接口 (ALI) 提供了新的人类生理模型. 这些模型克服了研究瘤病毒和癌症发病现有系统的局限性.

科学领域:

病毒学病毒学
在瘤学瘤学.
细胞生物学细胞生物学

背景情况:

大约20%的人类癌症与致癌病毒有关.
目前的实验模型在细胞产量,遗传修饰和瘤异质性方面存在局限性,阻碍了对病毒病原性和瘤发生的研究.
现有的癌细胞系不充分模拟自然病毒生命周期,表皮细胞的持久性和潜伏性,这些过程对于理解病毒媒介癌症发病至关重要.

研究的目的:

解决对可靠的人体生理细胞模型的需求,以研究病毒生命周期和癌症发病.
概述条件细胞重编程 (CCR) 和空气-液体接口 (ALI) 方法的应用.
为了建模宿主病毒相互作用和病毒介导的瘤发生.

主要方法:

利用条件细胞重编程 (CCR) 来快速建立从最小侵入性标本的细胞培养物.
在长期培养过程中保持细胞的血统功能和分化能力.
采用空气-液体接口 (ALI) 培养来促进上皮分化和回顾生理条件.

主要成果:

证明了能够在ALI区分CR细胞的建立.
展示了CR和ALI系统在模拟宿主病毒相互作用中的实用性.
验证了这些模型用于研究病毒媒介瘤发生的应用.

结论:

条件细胞重编程 (CCR) 与空气液体接口 (ALI) 培养相结合,提供了一个强大的人类生理模型系统.
这些先进的细胞培养技术克服了研究瘤病毒和癌症的传统模型的局限性.
在研究病毒生命周期,宿主病毒相互作用和病毒瘤发生机制方面,CR-ALI方法非常有价值.

关键词:

这是EBV的EBV.这是一个HPVHPVHPV.空气-液体接口接口有条件的细胞重编程.致癌病毒的致癌病毒.

更多相关视频

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

Published on: August 25, 2017

Generation of Organotypic Raft Cultures from Primary Human Keratinocytes

Generation of Organotypic Raft Cultures from Primary Human Keratinocytes

Published on: February 22, 2012

相关概念视频

Mechanisms of Retrovirus-induced Cancers

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...

Retrovirus Life Cycles

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...