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脂质转移蛋白Nir2和Nir3在细胞化过程中维持酸的信号传递和actin动态

Mayis Kaba^1,2, Amado Carreras-Sureda^1,2, Paula Nunes-Hasler³

¹Department of Cell Physiology and Metabolism, University of Geneva, Geneva, 1211, Switzerland.

Journal of cell science

|June 28, 2023

概括

酸氨基转移蛋白Nir2和Nir3维持酸氨基4,5-双酸在细胞杯中的稳态,这对于细胞复合过程中的actin重塑和细胞密封至关重要.

科学领域:

细胞生物学细胞生物学
生物化学生物化学
免疫学免疫学免疫学

背景情况:

消化细胞症涉及动态的actin细胞骨架改造,由酸和信号协调.
酸丁酸4,5-双酸盐[PI(4,5) P2]对于细胞杯的形成和关闭至关重要.

研究的目的:

为了研究酸丁氨基转移蛋白PITPNM1 (Nir2) 和PITPNM2 (Nir3) 在保持PI(4,5) P2在细胞形成过程中的稳态中所起的作用.
阐明Nir2和Nir3对动素收缩性,信号传递和细胞密封的影响.

主要方法:

通过CRISPR-Cas9基因编辑创建Nir2和Nir3淘汰细胞.
在细胞中分析Nir2和Nir3的表达.
测量血PI(4,5) P2水平,储存的入量 (SOCE) 和细胞效率.
分析了行为环的形成和胞细胞闭合的动态.

主要成果:

尼尔2和尼尔3聚集在细胞杯附近的内质网膜中.
丧失Nir2/3功能会减少血PI(4,5)P2,损害细胞化,并阻碍粒子捕获.
尼尔2/3的枯竭影响了actin环密度,并导致了堕胎的胞体关闭.
尼尔2或尼尔3的重新表达恢复了与PI (4,5) P2水平成比例的细胞化.

结论:

尼尔蛋白对于维护细胞杯中的PI{4,5) P2稳态至关重要.
这种氨酸维护维持了有效的胞细胞密封所需的活性重塑信号.
尼尔蛋白在细胞过程的动态调节中起着至关重要的作用.

关键词:

细胞信号传递细胞信号传递细胞骨架细胞骨架豁免权是一种豁免权.脂质转移是指脂质的转移.膜接触部位与膜的接触部位.

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