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人类腺病毒2型未被禁活的摄入通过Tetrahymena pyriformis状动物

Margot Olive¹, Jean Daraspe², Christel Genoud²

¹Laboratory of Environmental Chemistry, School of Architecture, Civil and Environmental Engineering (ENAC), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. tamar.kohn@epfl.ch.

Environmental science. Processes & impacts

|June 28, 2023

概括

状植物 Tetrahymena pyriformis 通过将病毒内部化,有效地从表面水中去除传染性人类腺病毒2型. 然而,病毒在摄入后仍然具有传染性,这表明病毒在水质方面发挥了双重作用.

科学领域:

环境微生物学环境微生物学
病毒学病毒学
亲生组织学亲生组织学亲生组织学

背景情况:

人类腺病毒是常见的表面水污染物.
原生体可以影响病毒的去除,但机制各不相同.
了解这些相互作用是评估水质的关键.

研究的目的:

为了研究人类腺病毒2型 (HAdV2) 和状虫Tetrahymena pyriformis之间的相互作用.
确定T. pyriformis. 通过HAdV2的去除动力学和机制.
评估与T. pyriformis.互动后的HAdV2的命运.

主要方法:

在淡水中同时化HAdV2和T. pyriformis.
在72小时内定量传染性HAdV2.
传输电子显微镜 (TEM) 用于可视化病毒内部化.

主要成果:

在72小时内,T. pyriformis实现了≥4 log10的传染性HAdV2的去除.
移除主要是由于内部化,而不是吸附或分泌的化合物.
在T. pyriformis食物真空中,TEM证实了HAdV2的存在.
在48小时内没有观察到HAdV2消化的证据.

结论:

甲 pyriformis 通过内部化有效地从水中去除传染性人类腺病毒2型.
T. pyriformis充当载体,在不降解感染病毒的情况下积累感染病毒.
这凸显了微生物在水质中的双重作用:从水柱中去除,但有可能导致病毒积累.

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