猫类莱什曼病:血液学和生物化学分析
Diogo Tiago da Silva1,2, Maria Luana Alves1,2, Júlio Cesar Pereira Spada1,2
1Laboratório de Medicina Veterinária Preventiva Aplicada, Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo - USP, Pirassununga, SP, Brasil.
概括
在猫中诊断猫类莱什曼尼病可能是具有挑战性的. 这项研究突出了关键的血液学和生化标志物,包括低血小板数和改变的蛋白质水平,有助于早期检测Leishmania婴儿感染.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 动物传染病 在动物中的传染病
- 猫科动物医学 猫科动物医学
背景情况:
- 莱什曼尼亚种类 (Leishmania spp.) 是一种 感染对猫来说是一个诊断挑战.
- 猫类莱什曼病的临床症状可能是非特异性的.
- 准确的诊断方法对于及时干预至关重要.
研究的目的:
- 评估Leishmania spp.的诊断方法. 在猫身上.
- 为了确定与Leishmania婴儿感染相关的血液学和生物化学变化.
- 在受感染的猫身上,将临床症状与实验室检测结果联系起来.
主要方法:
- 使用了酶相关免疫吸收试验 (ELISA),间接免疫光抗体试验 (IFAT),常规聚合酶链反应 (cPCR),定量PCR (qPCR) 和寄生虫学试验 (PA).
- ITS-1 PCR amplicons的测序证实了莱什曼尼亚婴儿.
- 对受感染和未受感染的猫进行了临床,血液学和生物化学分析.
主要成果:
- 在诊断测试中,患病率各不相同,IFAT显示的阳性率最高 (53.6%).
- 测序证实了Leishmania infantum作为致病剂.
- 与负面对照相比,受感染的猫的血小板数量显著降低,蛋白质过高和低蛋白质血症.
结论:
- 具有临床症状暗示莱什曼尼病的猫,并表现出特定的血液学 (血小板缩小) 和生化学 (高蛋白血症,低蛋白血症) 变化,需要对莱什曼尼菌种进行测试.
- 建议使用结合血清学和分子学方法的诊断方法.
- 早期检测和诊断对于在特有地区管理猫类莱什曼尼病至关重要.
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