通过基因组挖掘发现抗菌酸天然产品来自Bacillus velezensis
Jake Wilson1,2, Jerry Cui1, Toshiki Nakao1
1Department of Microbiology, The Ohio State University, Columbus, Ohio, USA.
Applied and environmental microbiology
|June 28, 2023
概括
研究人员从Bacillus velezensis中发现了两种新的酸,即酸胺E和F,它们来自Bacillus velezensis. 这些化合物对植物和人类病原体具有广泛的抗菌活性,为新的抗生素提供了潜力.
科学领域:
- 自然产品化学 自然产品化学
- 微生物学 微生物学
- 生物有机化学 生物有机化学
背景情况:
- 酸盐天然产品对于开发抗生素和农药至关重要.
- 虽然 Streptomyces 是已知的生产者,但其他细菌属具有显著的生物合成潜力.
- 生物信息调查表明,细菌中酸盐生物合成能力广泛存在,但尚未得到充分研究.
研究的目的:
- 为了研究未经探索的细菌种群的新酸天然产品.
- 从受污染的Bacillus velezensis基因组数据集中识别和描述新的酸盐化合物.
- 为了评估新发现的酸的抗菌活性.
主要方法:
- 细菌基因组的生物信息分析,以确定酸盐生物合成基因集群.
- 序列解卷以确定正确的生物体和基因群.
- 自然产品的隔离,结构阐明 (NMR,质谱) 和生物活性测定.
主要成果:
- 在Bacillus物种中发现了一个保存的酸盐生物合成基因集群,包括Bacillus velezensis.
- 两种含有l-phosphonoalanine的新型酸,酸胺E和F的分离和表征.
- 氨基酸E和F对植物病原体 (Erwinia rhapontici,Pantoea ananatis,Paenibacillus幼虫) 和其他细菌具有广泛的抗菌活性.
结论:
- 贝西路斯维莱泽尼斯产生具有显著抗菌性能的新型酸.
- 斯诺亚胺 E 和 F 是一种新型的酸盐天然产品,具有潜在的农业和医疗应用.
- 该研究强调了探索各种微生物种群对于发现天然产品和扩大对酸盐代谢知识的重要性.
相关概念视频
01:23Antimicrobial Proteins
1.1K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
1.1K
00:57Antibiotic Selection
54.8K
Overview
54.8K
01:28Biological Methods for Microbial Control
144
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
144
01:28Gene Regulation in Microbial Communities: Quorum Sensing
49
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
49