对宿主对病毒性感染的反应的综合时间尺度和多omics分析
Joan Márquez-Molins1,2, Pascual Villalba-Bermell1, Julia Corell-Sierra1
1Department of Molecular Interactions and Regulation, Institute for Integrative Systems Biology (I2SysBio), Consejo Superior de Investigaciones Científicas (CSIC), Universitat de València (UV), Parc Científic, Paterna, Spain.
Plant, cell & environment
|June 28, 2023
概括
跳跃特技病毒 (HSVd) 感染随着时间的推移重塑黄瓜的调节途径,最初改变基因表达,后来涉及表观遗传变化. 这影响了植物的防御机制和病原体的传播.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 病毒体是小的,圆形的RNA病原体,劫持植物的调节系统.
- 之前的研究经常在单个时间点或监管层面上检查病毒-宿主相互作用.
- 对病毒性感染的时间动态缺乏全面的了解.
研究的目的:
- 为了研究感染蜂突变病毒 (HSVd) 的黄瓜植物的全基因组时间变化.
- 在HSVd感染期间整合宿主转录组,sRNAnome和甲基组的分析.
- 阐明植物对病毒病原体产生反应的复杂,时间依赖的分子基础.
主要方法:
- 随着时间的推移,对受HSVd感染的黄瓜植物中的宿主转录组,sRNAnome和甲基组进行综合分析.
- 差异基因表达分析,包括外基因使用.
- 表观遗传分析以评估甲基化变化.
主要成果:
- 感染HSVd会导致黄瓜调节通路发生显著的,特定时间的变化.
- 早期感染涉及宿主转录组通过差异性外因子使用重新配置.
- 后期阶段显示由表观遗传修饰调节的逐步转录性下调,对内源性小RNA的影响有限.
结论:
- 在不同的感染阶段,HSVd感染动态重塑宿主调节网络.
- 关键受影响的途径包括植物防御,病原体运动限制和系统信号.
- 这项研究提供了HSVd感染期间调节性改变的第一个时间地图,有助于理解病毒-宿主相互作用.
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