结合机械映射和干扰对比显微镜,揭示了单细胞分化成巨细胞的粘弹性和粘附性特征
Mar Eroles1, Javier Lopez-Alonso2, Alexandre Ortega1
1Aix-Marseille University, INSERM, CNRS, LAI, Turing Centre for Living Systems, Marseille, France. felix.rico@inserm.fr.
Nanoscale
|June 28, 2023
概括
单细胞分化成巨细胞显著增加细胞硬性和粘附性. 这些机械变化,特别是在扩散巨细胞中,对于它们在炎症过程中的功能至关重要.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 生物物理学的生物物理.
- 免疫学 免疫学 免疫学
背景情况:
- 单细胞在炎症期间分化为巨细胞,这一过程涉及细胞机制和粘附的变化.
- 在单细胞与巨细胞分化过程中,粘性弹性和粘附的精确变化尚未完全理解.
研究的目的:
- 在分化成巨细胞过程中量化单细胞的形态,粘附和粘弹性质的变化.
- 阐明细胞力学在单细胞-巨细胞分化和随后的生物功能中的作用.
主要方法:
- 原子力显微镜 (AFM) 用于高分辨率的粘弹性映射.
- 干扰对比显微镜 (ICM) 用于单细胞分析.
- 定量全息断层扫描用于细胞形态评估.
主要成果:
- 单细胞分化成巨细胞导致细胞体积,表面积增加,并出现明显的圆和扩散亚群.
- 在分化巨细胞中,AFM显示出显著的硬化 (增加模量) 和固化 (减少流动性).
- 细胞硬度和固化的增加与较大的粘附区域相关,特别是在扩散巨细胞中,表明细胞骨重组.
结论:
- 单细胞分化成巨细胞涉及粘弹性和粘附性质的重大变化.
- 这些机械特征,包括增加的刚性和固化,对于炎症环境中的巨细胞功能至关重要.
- 这些发现表明,细胞骨在分化过程中的重组会使巨细胞为节能机械敏感活动做好准备.
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