德SRNA诱导的免疫力准了等离子体,并被病毒运动蛋白抑制
Caiping Huang1, Ana Rocío Sede1, Laura Elvira-González1
1Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67000 Strasbourg, France.
The Plant cell
|June 28, 2023
概括
双链RNA (dsRNA) 通过在等离子体上沉积来触发植物免疫力,从而限制病毒的运动. 病毒通过抑制dsrna诱导的质沉积来抵制这种防御,突出显示了植物病毒的关键战场.
科学领域:
- 植物免疫和病毒学
- 分子植物微生物相互作用.
背景情况:
- 双链RNA (dsRNA) 已知用于抗病毒RNA沉默.
- dsRNA在植物中也会引起模式触发免疫 (PTI),但其信号通路的理解很少.
- 了解dSRNA诱导的PTI对于开发新的抗病毒策略至关重要.
研究的目的:
- 阐明植物中dRNA诱导的PTI的作用模式和信号通路.
- 调查dsrna在限制病毒感染进展中的作用.
- 为了确定参与dsrna介导的植物防御的关键分子组件.
主要方法:
- 在Arabidopsis thaliana和Nicotiana benthamiana中使用多色体内成像.
- 进行了GFP移动性,色染色和等离子体标记线的分析.
- 研究了特定的激酶模块,蛋白质和信号的参与.
主要成果:
- 德SRNA诱导的PTI通过触发质沉积在等离子体上来限制病毒感染.
- 这种质沉积限制了宏分子运输,阻碍了病毒细胞间传播.
- 确定了关键参与者:SOMATIC胚胎发生受体类似的基因酶1,BOTRYTIS诱导的基因酶1/AVRPPHB易受性1-类似的基因酶1,PlasMODESMATA定位的蛋白质1/2/3,CALMODULIN-LIKE 41,以及Ca2+信号.
- 与细菌诱导者不同的是,dsRNA诱导的PTI不会触发反应性氧物种 (ROS) 爆发.
- 病毒运动蛋白抑制dRNA诱导的质沉积,以促进感染.
结论:
- 植物免疫信号通过dRNA诱导的质沉积在等离子体上限制病毒的运动.
- 病毒已经进化了机制 (抑制沉积) 来抵消这种植物防御.
- 这项研究揭示了独特的PTI途径和病毒对策略,进步了我们对植物病毒相互作用的理解.
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