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Circ_0011373通过调节miR-1271/LRP6轴促进乳头甲状腺癌的进展
Guoxiang Huang1, Lijun Mao2, Xiarong Hu3
1The First Department of General Surgery, Affiliated Dongguan People's Hospital, Southern Medical University(Dongguan People's Hospital), Dongguan, Guangdong, China.
概括
循环RNA (circ) 0011373通过微RNA (miR) -1271轴通过升调脂蛋白受体相关蛋白6 (LRP6) 来促进乳头甲状腺癌 (PTC) 的进展. 抑制circ 0011373可能为PTC提供治疗策略.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 在RNA生物学,RNA生物学.
背景情况:
- 乳头甲状腺癌 (PTC) 是最常见的甲状腺癌类型.
- 了解PTC进展背后的分子机制对于开发有效的治疗方法至关重要.
- 循环RNAs (circRNAs) 已经成为各种癌症 (包括PTC) 的重要调节者.
研究的目的:
- 为了研究circ_0011373在皮肤状甲状腺癌中的调节作用.
- 阐明涉及PTC中的circ_0011373,microRNA (miR) -1271和脂蛋白受体相关蛋白6 (LRP6) 的分子机制.
- 探索针对PTC疗法的circ_0011373/miR-1271/LRP6轴的潜力.
主要方法:
- 定量实时PCR (qRT-PCR) 用于评估circ_0011373,miR-1271和LRP6mRNA的表达水平.
- 细胞检测包括细胞循环和细胞亡的流动细胞计,以及迁移和入侵的跨井检测.
- 双化酶报告员和RIP测定以确认分子之间的直接相互作用.
- 西方斑分析用于评估蛋白质表达.
- 在体内异种移植瘤模型验证circ_0011373在瘤生长中的作用.
主要成果:
- 在PTC组织和细胞系中,Circ_0011373和LRP6的调高显著,而miR-1271的调低显著.
- Knockdown of circ_0011373 抑制了 PTC 细胞的增殖,迁移和入侵,同时促进了细胞亡.
- Circ_0011373与miR-1271直接相互作用,这反过来又针对了LRP6.6的目标. Circ_0011373 积极调节的 LRP6 表达式.
- 过度表达miR-1271通过调节LRP6抑制了PTC细胞的进展,而circ_0011373敲击抑制了体内瘤的生长.
结论:
- 通过调节miR-1271/LRP6轴,Circ_0011373促进了PTC进展.
- 这种circ_0011373/miR-1271/LRP6通路代表了皮肤状甲状腺癌的潜在治疗标.
- 针对circ_0011373可能是一个有希望的策略来抑制PTC的发展.
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