确定确定纳米颗粒血液循环时间的蛋白质
Cintia Marques1,2, Mohammad Javad Hajipour3, Célia Marets4
1Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland.
ACS nano
|June 28, 2023
概括
中性和正电荷的超偏磁铁氧化物纳米粒子 (SPIONs) 呈现出不同的循环时间. 特定的蛋白质吸附,除了松素,决定了纳米粒子的生物分布和治疗潜力.
科学领域:
- 纳米医学是一种纳米医学.
- 生物材料科学 生物材料科学
- 药理动力学 药理动力学
背景情况:
- 纳米粒子 (NP) 的治疗效果和毒性与系统循环时间有关.
- 在NP上吸附的蛋白质 (冠状元) 影响它们的血半衰期.
- 识别关键的冠状蛋白对于控制NP生物分布至关重要.
研究的目的:
- 分析超偏磁氧化铁纳米粒子 (SPIONs) 的 in vivo循环时间和冠状结构.
- 调查SPION表面电荷/化学对循环时间和蛋白质吸附的影响.
- 为了确定决定NP循环寿命的特定蛋白质.
主要方法:
- 具有不同表面电荷的SPIONs的系统循环时间在体内被测量.
- 随着时间的推移,使用蛋白质组学技术分析了SPIONs的冠状病毒成分.
- 建立了蛋白质吸附概况和循环半衰期之间的相关性.
主要成果:
- 中性电荷的SPION显示出最长的循环时间,而正电荷的SPION显示出最短的循环时间.
- 类似的opsonin/dysopsonin含量并不总是与循环时间相关,这表明涉及其他因素.
- 长期循环的SPION吸收了更高水平的特定蛋白质,包括骨质蛋白和阿波利波蛋白CI.
- 短循环的SPIONs显示出血红蛋白吸附的增加.
结论:
- SPION表面电荷显著影响了in vivo循环时间.
- 特定的蛋白质吸附概况,超出一般的松素/松素水平,是NP循环时间的关键决定因素.
- 像骨质素,脂蛋白脂酶和血红蛋白这样的蛋白质可以作为预测NP循环寿命的关键生物标志物.
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