相关实验视频
Updated: Jul 25, 2025

07:00
In Ovo Intravascular Injection in Chicken Embryos
Published on: June 3, 2022
6.1K
构成性活性的pSMAD2/3相对改善了原始生殖细胞的增殖
Masumeh Zare1, Seyed Ziaeddin Mirhoseini1, Shahrokh Ghovvati1
1Department of Animal Sciences, Faculty of Agriculture, University of Guilan, Rasht, Guilan, Iran.
Molecular reproduction and development
|June 28, 2023
概括
转录因子SMAD2/3促进原生殖细胞 (PGC) 的增殖. 激活SMAD2/3显著增强了PGC扩张,为鸟类PGC培养提供了一种新方法.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
背景情况:
- 原始生殖细胞 (PGC) 对于繁殖和物种的保存至关重要.
- 有效培养PGC对研究,保护和生物技术至关重要.
- SMAD2/3转录因子是已知的基因调节者,但它们在PGC增殖中的作用尚未被探索.
研究的目的:
- 研究TGF-β信号传递和SMAD2/3对PGC增殖的影响.
- 确定SMAD2/3激活是否可以增强长期PGC培养.
主要方法:
- PGCs (阶段26-28 HH) 被分离和培养.
- 细胞接受了TGF-β信号传导激动剂 (IDE1,Activin-A) 和对抗剂 (SB431542) 的治疗.
- 用构成性活性的SMAD2/3 (SMAD2/3CA) 转化PGC,并分析其增殖和基因表达.
主要成果:
- TGF-β激动剂部分改善了PGC增殖;对抗剂抑制了它.
- 用SMAD2 / 3CA感染显著增强了PGC的扩散超过5周.
- 过度表达的SMAD2/3CA与多能性基因NANOG,OCT4和SOX2.2相互作用.
结论:
- 在的PGC增殖中,SMAD2/3信号发挥着积极的作用.
- 构成性活跃的SMAD2/3 (SMAD2/3CA) 能够有效和长时间扩展鸟类PGCs.
- 这一发现为鸟类PGC培养和相关应用提供了有前途的战略.
相关概念视频
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Mitogens and the Cell Cycle
6.6K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.6K
Negative Regulator Molecules
35.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.5K
Methods of Nuclear Reprogramming
1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K

