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基于纸质的电化学免疫传感器用于测定对称的二甲基氨酸

Nadine Wenninger¹, Sudkate Chaiyo², Alexander Kollau³

¹Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Schubertstraße 1, Graz, 8010, Austria.

Biosensors & bioelectronics

|June 28, 2023

概括

我们开发了一种新型的免疫传感器,用于检测尿液中的对称二甲基氨酸 (SDMA),这是病的关键生物标志物. 该工具提供敏感和选择性的SDMA测量,以改善病诊断和监测.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
临床诊断临床诊断临床诊断

背景情况:

对称二甲基氨酸 (SDMA) 是病的新兴生物标志物,因为随着功能下降,其尿液分泌量减少.
血中SDMA含量升高表明病,小动物实践已建立参考范围.
在尿液中监测SDMA为诊断和管理功能障碍提供了潜在的途径.

研究的目的:

开发一种直接,选择性和敏感的免疫传感器,用于确定尿液中的SDMA.
建立一个基于纸张的电化学传感平台,用于SDMA量化.
为了验证免疫传感器在人类尿样中的性能和适用性.

主要方法:

一种基于纸张的电化学免疫传感器,利用抗SDMA抗体进行针对性的SDMA检测.
基于因免疫复合体形成干扰电子转移而导致的氧化还原指标信号减少的量化.
方波电压测量用于信号测量和分析.

主要成果:

免疫传感器表明,峰值下降和SDMA度从50nM到1μM之间存在线性相关性.
SDMA的低检测极限达到了15nM.
传感器表现出极好的选择性,在常见的生理干扰的情况下没有显著的信号减少.
在从健康个体获得的人类尿样中量化SDMA的成功应用.

结论:

开发的免疫传感器提供了一种敏感和选择性的方法,用于尿液中的SDMA测定.
这项技术对脏疾病的非侵入性诊断和监测具有前景.
基于尿液的SDMA监测可以在临床实践中成为评估脏健康的宝贵工具.

关键词:

抗体是对抗体的一种.生物传感器是一种生物传感器.石墨烯是一种石墨烯.脏疾病是一种脏疾病.方形波电压测量方形波电压测量

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