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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
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相关实验视频

Updated: Jul 25, 2025

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
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QKI将内部的m7转录转化为压力颗粒并调节mRNA代谢

Zhicong Zhao1, Ying Qing2, Lei Dong2

  • 1Department of Systems Biology, Beckman Research Institute of City of Hope, Monrovia, CA 91016, USA; Department of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Cell
|June 28, 2023
PubMed
概括

震动蛋白 (QKI) 将内部的N7-甲基瓜诺辛 (m7G) 修饰物与信使RNA (mRNA) 结合起来. 这种相互作用调节mRNA的稳定性和翻译,影响癌细胞的化疗敏感性.

关键词:
G3BP1 其他METTL1 时间甲基氨酸在QKI药物耐药性其他国家mRNA代谢mRNA 稳定性压力颗粒翻译法规

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科学领域:

  • 分子生物学
  • RNA 修改
  • 癌症研究

背景情况:

  • 对于mRNA处理和蛋白质合成至关重要的是N7-甲基氨酸 (m7G) 的修饰,主要是在5'顶部.
  • 在信使RNAs (mRNAs) 中的内部m7G修饰的功能意义仍然在很大程度上未被探索.

研究的目的:

  • 研究内部m7G修饰在mRNA代谢中的作用.
  • 识别识别和结合内部m7G修饰的mRNA的蛋白质.
  • 阐明这种相互作用在细胞应激反应和药物耐药性的作用.

主要方法:

  • 内部m7G甲基组的全转录组概况和映射.
  • 在m7G修饰的mRNA上识别和描述Quaking蛋白 (QKI) 的结合点.
  • 共同免疫沉试验用于研究蛋白质与蛋白质的相互作用 (QKI7和G3BP1).
  • 在压力条件下分析mRNA稳定性和翻译效率.

主要成果:

  • 鉴定出超过1,000个具有高可信度的内部m7G修饰和QKI结合的mRNA标,具有保留的"GANGAN"动机.
  • 震动蛋白 (QKI) 选择性地识别和结合内部m7G修饰的mRNA.
  • QKI7与压力颗粒核心蛋白G3BP1相互作用,并促进m7G修饰的转录物转移为压力颗粒.
  • 这一过程调节了mRNA的稳定性和翻译,特别是减弱了Hippo信号通路基因的翻译,从而使癌细胞对化疗敏感.

结论:

  • QKI作为mRNA上的内部m7G修饰的读取器.
  • 内部m7G修饰和QKI结合在mRNA代谢和细胞应激反应中起着重要作用.
  • QKI-QKI7-G3BP1轴调节mRNA的翻译和稳定性,影响癌细胞的耐药性和敏感性.