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通过IgE介导的聚乙烯糖醇和/或聚酸盐的内型化 80 过敏
Toon Ieven1, Lieve Coorevits1, Martijn Vandebotermet2
1KU Leuven Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, KU Leuven, Leuven, Belgium; Department of General Internal Medicine, Division of Allergy and Clinical Immunology, University Hospitals Leuven, Leuven, Belgium.
患有聚乙烯糖醇 (PEG) 或聚酸盐80 (PS80) 过敏的患者可以安全地接种SARS-CoV-2疫苗. 反应性与IgE识别短PEG动机有关,而不仅仅是PS80,并且脂质纳米粒子疫苗耐受性良好.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 过敏研究 研究过敏
背景情况:
- 聚乙烯甘醇 (PEG) 和聚酸盐80 (PS80) 的过敏会限制SARS-CoV-2疫苗接种.
- 交叉活性和PEG分子重量依赖的机制尚未完全理解.
研究的目的:
- 评估PEG或PS80过敏患者对PEG化脂质纳米粒子 (LNP) 疫苗的耐受性.
- 研究这些过敏患者群体的免疫反应机制.
主要方法:
- 包括患有双重PEG/PS80过敏 (n=3),PEG单一过敏 (n=7) 和PS80单一过敏 (n=2) 的患者.
- 使用PEG,PS80,BNT162b2和PEG化脂质进行了分级的疫苗挑战和基激活测试 (wb-BAT/allo-BAT).
- 在患者和对照中测量血清PEG特异性IgE水平.
主要成果:
- BNT162b2 (Pfizer-BioNTech) 疫苗在双重和PEG单种过敏患者中耐受良好,诱导抗尖峰IgG.
- PS80单种过敏患者可以耐受单剂量BNT162b2.2.
- 在双重和PEG单种过敏患者中观察到含PEG抗原的基激活,BNT162b2的激活率最高,并且是IgE介导的.
- 主要检测到PEG特异性的IgE是在双重过敏患者中.
结论:
- PEG和PS80之间的交叉反应是由IgE识别短PEG动机的介导;PS80单种过敏是PEG独立的.
- 在LNP中球形PEG暴露增加了基细胞激活的敏感性.
- 所有患有PEG和/或PS80过敏的患者都可以安全地接种SARS-CoV-2疫苗.
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