对立的神经和神经递质信号的电路协调
Marta E Soden1, Joshua X Yee2, Larry S Zweifel3,4
1Department of Pharmacology, University of Washington, Seattle, WA, USA. msoden@uw.edu.
Nature
|June 28, 2023
概括
神经元同时释放具有相反作用的神经递质和神经. 这项研究揭示了GABA和神经激素信号如何协调以控制多巴胺神经元活动并优化行为.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 神经元从不同的囊泡共同释放快速的神经递质和缓慢的神经.
- 共同释放物质对抗信号 (刺激/抑制) 如何控制神经回路的机制尚不清楚.
- 之前的研究缺乏这些信号通路的细胞和电路特定隔离方法.
研究的目的:
- 为了研究如何同时释放的神经递质和神经与相反的行动协调神经电路输出.
- 开发一种方法,同时操纵神经递质和神经基因在不同类型的细胞中,跨大脑区域.
主要方法:
- 开发了一种基于基因的解剖切断程序,使用不同的DNA重组酶.
- 利用CRISPR-Cas9突变发生,独立准神经递质和神经基因.
- 同时操纵两个大脑区域 (侧向下丘脑和腹部体区域) 的不同细胞类型的基因.
主要成果:
- 证明横向下丘脑神经元共同产生神经素 (刺激性) 和GABA (抑制性) 协调腹膜区域多巴胺神经元的激活.
- 显示GABA抑制局部GABA神经元,消毒多巴胺神经元 (快速升).
- 透露的神经素通过Nsr1直接在多巴胺神经元中产生缓慢的信号,增强行为反应.
结论:
- 具有相反信号的神经递质 (GABA) 和神经 (神经) 可以分别起作用来控制神经输出.
- 这些信号在不同的时间尺度和通过不同的细胞类型运行,以优化电路功能.
- 这种协调的信号增强了行为反应,证明了神经控制的新机制.
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