相关实验视频

Updated: Jul 25, 2025

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Published on: March 3, 2023

在人类糖尿病足中绘制细胞衰老网络

Grace T Yu¹, Dileep D Monie¹, Sundeep Khosla^2,3

¹Mayo Clinic Medical Scientist Training Program, Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic Alix School of Medicine, Rochester, MN, USA.

|June 28, 2023

概括

细胞衰老通过改变基因表达和蛋白质相互作用,导致糖尿病足. 像p53和p21这样的关键调节者与这些慢性伤口的病原发生有关.

科学领域:

细胞和分子生物学细胞和分子生物学
伤口治愈研究研究伤口治愈研究
糖尿病并发症糖尿病并发症

背景情况:

细胞衰老,或不可逆转的细胞循环停止,与慢性与年龄有关的疾病有关.
它在糖尿病足 (DFU) 病变发生中的特定作用尚未完全理解.
DFU是糖尿病的重要并发症,通常以不良的愈合为特征.

研究的目的:

调查老化的表型对糖尿病足的发展的贡献.
在DFU组织中分析基因表达和蛋白质-蛋白质相互作用网络.

主要方法:

差异基因表达分析使用DFU伤口边缘和未涉及皮肤的散装RNA测序.
使用NetDecoder进行网络分析,以识别特定环境的蛋白质与蛋白质相互作用.
统计分析包括Wald测试与本雅米尼-霍赫伯格校正.

主要成果:

衰老标志物 (CDKN1A,CXCL8,IGFBP2,IL1A,MMP10,SERPINE1,TGFA) 的上调和DFU中TP53的下调.
在DFU中扰乱了蛋白质-蛋白质相互作用网络,抑制相互作用减少.
在DFU形成中,TP53 (p53) 和CDKN1A (p21) 被确定为关键调节器.

结论:

细胞衰老是糖尿病足病变的重要媒介.
与衰老相关的基因表达和蛋白质相互作用的改变有助于DFU的发展.
针对细胞衰老路径可能为DFU提供治疗策略.

关键词:

细胞衰老细胞衰老糖尿病足部糖尿病足部网络分析网络分析皮肤老化皮肤老化伤口愈合伤口愈合

更多相关视频

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

相关概念视频

Pathophysiology of Diabetes

Pathophysiology of Diabetes

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...

Tissue Renewal without Stem Cells

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...