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通过ARTC1介导的VAPBADP-ribosylation调节的平衡

Xueyao Ma¹, Mengyuan Li¹, Yi Liu¹

¹College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding 071002, China.

Journal of molecular cell biology

|June 29, 2023

概括

单-ADP-ribosylation (MARylation) 调节了关键的细胞过程. 这项研究表明,人类的ARTC1自我稳定并修改VAPB,这对于维持平衡至关重要.

科学领域:

生物化学生物化学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

单-ADP-ribosylation (MARylation) 是一种关键的翻译后修饰,可以调节多种细胞功能.
包括人类ARTC家族在内的ADP-核糖转移酶 (ARTs) 催化MARylation.
人类ARTC家族包括四个成员,其中hARTC1和hARTC5具有酶活性.

研究的目的:

研究人类ARTC家族的同质性,表达和局部化,重点关注hARTC1.
阐明hARTC1在细胞过程中的调节机制和功能作用.

主要方法:

对hARTC家族成员的同质性,表达和局部化模式的系统检查.
在hARTC家族成员之间的相互作用研究.
使用生物化学分析识别和描述hARTC1点.
通过敲击实验和细胞平衡的评估,对hARTC1活动的功能分析.

主要成果:

hARTC3与hARTC1相互作用并稳定,增强其酶活性.
囊泡相关膜蛋白相关蛋白B (VAPB) 被确定为hARTC1的新目标,Arg50是ADP-ribosylation位点.
抑制hARTC1破坏了细胞内平衡,这表明它在信号调节中的作用.

结论:

hARTC1通过Arg50.0的VAPB的ADP-ribosylation调节细胞内平衡,在调节细胞内平衡方面发挥着重要作用.
与hARTC3的相互作用有助于hARTC1的稳定和活性.
这项研究确定了hARTC1的新ER本地化目标,并强调了它在信号通路中的重要性.

关键词:

在ATRC中,我们可以使用ATRC.这就是VAPB.的恒常性是的恒常性.在HARTC1中,一个ADP-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation-ribosylation.

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