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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
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脉冲刺激布里卢恩显微镜的显微镜.

Desmond M Chow, Seok-Hyun Yun

    Optics express
    |June 29, 2023
    PubMed
    概括

    脉冲刺激布里卢恩散射 (SBS) 与自发的布里卢恩散射相比,在生物样本分析中提供了更高的信号噪声比率 (SNR). 这一进步使得具有较低平均功率的高分辨率机械性能映射成为可能.

    科学领域:

    • 生物物理学的生物物理.
    • 光学显微镜的使用方法
    • 材料科学 材料科学 材料科学

    背景情况:

    • 刺激布里卢恩散射 (SBS) 是一种非线性光学技术,用于探测生物样品的机械性质.
    • 对于SBS,通常需要高光学强度,由于潜在的光损伤,对生物应用构成挑战.
    • 达到足够的信号噪声比 (SNR) 对于准确的测量至关重要.

    研究的目的:

    • 为了证明脉冲SBS可以在可比的平均功率水平下实现比自发Brillouin散射 (SpBS) 更高的SNR.
    • 开发和验证一种适用于生物样本分析的新型脉冲SBS方案.
    • 为了使生物样本中的机械性质能够以高分辨率绘制地图.

    主要方法:

    • 实施一种新型脉冲SBS方案,利用低工作周期,纳米秒脉冲用于和探针束.
    • 使用不同整合时间和平均功率水平的水样测量SNR.
    • 从体外细胞样本中获取Brillouin频率偏移,线宽和增益幅度的高分辨率地图.

    主要成果:

    • 在水样中,通过平均功率为10 mW (2 ms整合) 和50 mW (200 μs整合) 达到超过1000的射击噪声限制SNR.
    • 高分辨率的机械性质图 (频率转移,线宽,增强幅度) 由细胞在体外获得,光谱获取时间为20毫秒.

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  • 该研究验证了有关脉冲SBS增强SNR的理论预测.

    • 结论:

    • 脉冲刺激的Brillouin散射在生物成像中的SNR方面明显优于自发的Brillouin散射.
    • 开发的脉冲SBS技术适用于生物样本的高分辨率,无损的机械表征.
    • 这一进步为细胞生物学和相关领域的定量机械探测开辟了新的途径.